Next Article in Journal
Emerging Viruses in the Felidae: Shifting Paradigms
Previous Article in Journal
Clinical Characteristics and Genetic Variability of Human Rhinovirus in Mexico
Open AccessReview

Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis

1
School of Veterinary Science, The University of Queensland, Gatton, QLD 4343, Australia
2
Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St Lucia, QLD 4072, Australia
3
Queensland Agricultural Biotechnology Facility, Ritchie Laboratories, St Lucia, QLD 4067, Australia
*
Author to whom correspondence should be addressed.
Received: 20 December 2011 / Revised: 26 January 2012 / Accepted: 30 January 2012 / Published: 3 February 2012
Bacterial artificial chromosome (BAC) vectors were first developed to facilitate the propagation and manipulation of large DNA fragments in molecular biology studies for uses such as genome sequencing projects and genetic disease models. To facilitate these studies, methodologies have been developed to introduce specific mutations that can be directly applied to the mutagenesis of infectious clones (icBAC) using BAC technologies. This has resulted in rapid identification of gene function and expression at unprecedented rates. Here we review the major developments in BAC mutagenesis in vitro. This review summarises the technologies used to construct and introduce mutations into herpesvirus icBAC. It also explores developing technologies likely to provide the next leap in understanding these important viruses. View Full-Text
Keywords: chromosomes; artificial; bacterial; recombination; genetic; mutagenesis; cloning; molecular methods; transposition; DNA viruses; infectious clone chromosomes; artificial; bacterial; recombination; genetic; mutagenesis; cloning; molecular methods; transposition; DNA viruses; infectious clone
Show Figures

Figure 1

MDPI and ACS Style

Hall, R.N.; Meers, J.; Fowler, E.; Mahony, T. Back to BAC: The Use of Infectious Clone Technologies for Viral Mutagenesis. Viruses 2012, 4, 211-235.

Show more citation formats Show less citations formats

Article Access Map by Country/Region

1
Only visits after 24 November 2015 are recorded.
Back to TopTop