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Article

Engineered Disease Resistance in Cotton Using RNA-Interference to Knock down Cotton leaf curl Kokhran virus-Burewala and Cotton leaf curl Multan betasatellite Expression

1
Center of Excellence in Molecular Biology, University of the Punjab, 87 W Canal Bank Road, Thokar Niaz Baig, Lahore 53700, Pakistan
2
Institute of Agricultural Sciences (IAGS), University of the Punjab, Lahore 54590, Pakistan
3
Jamie Whitten Delta States Research Center, United States Department of Agriculture (USDA), Stoneville, MS 38776, USA
4
School of Plant Sciences, University of Arizona, Tucson, AZ 85721, USA
*
Authors to whom correspondence should be addressed.
Academic Editor: Gian Paolo Accotto
Received: 20 June 2017 / Revised: 8 August 2017 / Accepted: 30 August 2017 / Published: 14 September 2017
(This article belongs to the Special Issue Geminiviruses)
Cotton leaf curl virus disease (CLCuD) is caused by a suite of whitefly-transmitted begomovirus species and strains, resulting in extensive losses annually in India and Pakistan. RNA-interference (RNAi) is a proven technology used for knockdown of gene expression in higher organisms and viruses. In this study, a small interfering RNA (siRNA) construct was designed to target the AC1 gene of Cotton leaf curl Kokhran virus-Burewala (CLCuKoV-Bu) and the βC1 gene and satellite conserved region of the Cotton leaf curl Multan betasatellite (CLCuMB). The AC1 gene and CLCuMB coding and non-coding regions function in replication initiation and suppression of the plant host defense pathway, respectively. The construct, , was transformed into cotton plants using the Agrobacterium-mediated embryo shoot apex cut method. Results from fluorescence in situ hybridization and karyotyping assays indicated that six of the 11 T1 plants harbored a single copy of the Vβ transgene. Transgenic cotton plants and non-transgenic (susceptible) test plants included as the positive control were challenge-inoculated using the viruliferous whitefly vector to transmit the CLCuKoV-Bu/CLCuMB complex. Among the test plants, plant Vβ-6 was asymptomatic, had the lowest amount of detectable virus, and harbored a single copy of the transgene on chromosome six. Absence of characteristic leaf curl symptom development in transgenic Vβ-6 cotton plants, and significantly reduced begomoviral-betasatellite accumulation based on real-time polymerase chain reaction, indicated the successful knockdown of CLCuKoV-Bu and CLCuMB expression, resulting in leaf curl resistant plants. View Full-Text
Keywords: Begomovirus; cotton leaf curl disease; Rep protein; siRNA; transgenic resistance Begomovirus; cotton leaf curl disease; Rep protein; siRNA; transgenic resistance
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MDPI and ACS Style

Ahmad, A.; Zia-Ur-Rehman, M.; Hameed, U.; Qayyum Rao, A.; Ahad, A.; Yasmeen, A.; Akram, F.; Bajwa, K.S.; Scheffler, J.; Nasir, I.A.; Shahid, A.A.; Iqbal, M.J.; Husnain, T.; Haider, M.S.; Brown, J.K. Engineered Disease Resistance in Cotton Using RNA-Interference to Knock down Cotton leaf curl Kokhran virus-Burewala and Cotton leaf curl Multan betasatellite Expression. Viruses 2017, 9, 257. https://0-doi-org.brum.beds.ac.uk/10.3390/v9090257

AMA Style

Ahmad A, Zia-Ur-Rehman M, Hameed U, Qayyum Rao A, Ahad A, Yasmeen A, Akram F, Bajwa KS, Scheffler J, Nasir IA, Shahid AA, Iqbal MJ, Husnain T, Haider MS, Brown JK. Engineered Disease Resistance in Cotton Using RNA-Interference to Knock down Cotton leaf curl Kokhran virus-Burewala and Cotton leaf curl Multan betasatellite Expression. Viruses. 2017; 9(9):257. https://0-doi-org.brum.beds.ac.uk/10.3390/v9090257

Chicago/Turabian Style

Ahmad, Aftab, Muhammad Zia-Ur-Rehman, Usman Hameed, Abdul Qayyum Rao, Ammara Ahad, Aneela Yasmeen, Faheem Akram, Kamran S. Bajwa, Jodi Scheffler, Idrees A. Nasir, Ahmad A. Shahid, Muhammad J. Iqbal, Tayyab Husnain, Muhammad S. Haider, and Judith K. Brown 2017. "Engineered Disease Resistance in Cotton Using RNA-Interference to Knock down Cotton leaf curl Kokhran virus-Burewala and Cotton leaf curl Multan betasatellite Expression" Viruses 9, no. 9: 257. https://0-doi-org.brum.beds.ac.uk/10.3390/v9090257

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