Next Article in Journal
Novel Population Pharmacokinetic Approach to Explain the Differences between Cystic Fibrosis Patients and Healthy Volunteers via Protein Binding
Previous Article in Journal
Ucuùba (Virola surinamensis) Fat-Based Nanostructured Lipid Carriers for Nail Drug Delivery of Ketoconazole: Development and Optimization Using Box-Behnken Design
Article

Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs

1
Department of Drug Sciences, University of Pavia, 27100 Pavia, Italy
2
Pharmaexceed S.r.l., 27100 Pavia, Italy
3
Environmental Research Center, ICS MAUGERI SPA SB, Institute of Pavia, IRCCS, 27100 Pavia, Italy
4
Department of Biomedical, Surgical and Dental Sciences, University of Milan, 20100 Milan, Italy
5
Department of Cerebrovascular Diseases, Fondazione IRCCS Istituto Neurologico Carlo Besta, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 29 April 2019 / Revised: 14 June 2019 / Accepted: 14 June 2019 / Published: 17 June 2019
The aim of this work was to load an anticancer drug, paclitaxel (PTX), on Silk Fibroin Nanoparticles (SFNs) by using an exogenous approach. SFNs were produced, freeze-dried and then loaded with PTX. An exogenous method allowed us to reduce both drug loss and environmental impact. In order to quantify PTX loaded in SFNs, a simple and reliable method using reversed phase liquid chromatography coupled to tandem mass spectrometry (rp-UHPLC-MS/MS) was developed. This methodology was validated by the determination of spiked QC samples in three consecutive days. Good accuracy and precision of the method were obtained, while the intra-day and inter-day precisions were less than 10.3%. For PTX, the limit of quantitation (LOQ) was 5.0 ng/mL. Recovery from the matrix (SFNs-PTX pellets) was calculated (81.2% at LOQ value) as PTX was entrapped in a new matrix like the polymer silk fibroin-based. This method was successfully applied to determine the encapsulation efficiency (1.00 ± 0.19%) and the nanoparticle loading (0.12 ± 0.02% w/w). The in vitro anticancer activity of SFNs-PTX was tested against CFPAC-1 cancer cells; results demonstrated a very high cytotoxic activity of SFNs-PTX, with a dose dependent inhibition of CFPAC-1 proliferation, confirmed by the IC50 value of 3450 ± 750 ng/mL. View Full-Text
Keywords: silk fibroin; nanoparticles; paclitaxel; UHPLC-MS/MS; method validation silk fibroin; nanoparticles; paclitaxel; UHPLC-MS/MS; method validation
Show Figures

Graphical abstract

MDPI and ACS Style

Perteghella, S.; Sottani, C.; Coccè, V.; Negri, S.; Cavicchini, L.; Alessandri, G.; Cottica, D.; Torre, M.L.; Grignani, E.; Pessina, A. Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs. Pharmaceutics 2019, 11, 285. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics11060285

AMA Style

Perteghella S, Sottani C, Coccè V, Negri S, Cavicchini L, Alessandri G, Cottica D, Torre ML, Grignani E, Pessina A. Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs. Pharmaceutics. 2019; 11(6):285. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics11060285

Chicago/Turabian Style

Perteghella, Sara, Cristina Sottani, Valentina Coccè, Sara Negri, Loredana Cavicchini, Giulio Alessandri, Danilo Cottica, Maria L. Torre, Elena Grignani, and Augusto Pessina. 2019. "Paclitaxel-Loaded Silk Fibroin Nanoparticles: Method Validation by UHPLC-MS/MS to Assess an Exogenous Approach to Load Cytotoxic Drugs" Pharmaceutics 11, no. 6: 285. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics11060285

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop