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Article

Perillaldehyde 1,2-epoxide Loaded SLN-Tailored mAb: Production, Physicochemical Characterization and In Vitro Cytotoxicity Profile in MCF-7 Cell Lines

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Department of Pharmaceutical Technology, Faculty of Pharmacy (FFUC), University of Coimbra, Pólo das Ciências da Saúde, Azinhaga de Santa Comba, 3000-548 Coimbra, Portugal
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CEB—Centre of Biological Engineering, University of Minho, Campus de Gualtar 4710-057 Braga, Portugal
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Department of Endocrinology, Hospital de São João, Alameda Prof. Hernâni Monteiro, 4200-319 Porto, Portugal
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CREA-Research Centre for Food and Nutrition, Via Ardeatina 546, 00178 Rome, Italy
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Department of Pharmacy, University of Napoli Federico II, 80131 Napoli, Italy
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Department of Technique and Food Product Development, Warsaw University of Life Sciences (WULS-SGGW) 159c Nowoursynowska, 02-776 Warsaw, Poland
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Institute of Neurobiology, Bulgarian Academy of Sciences, 23 Acad. G. Bonchev str., 1113 Sofia, Bulgaria
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Institute of Genetics and Animal Breeding, Polish Academy of Sciences, Jastrzębiec, 05-552 Magdalenka, Poland
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Department of Pharmacognosy, University of Vienna, Althanstraße 14, 1090 Vienna, Austria
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Ludwig Boltzmann Institute for Digital Health and Patient Safety, Medical University of Vienna, Spitalgasse 23, 1090 Vienna, Austria
11
Laboratory of Nanotechnology and Nanomedicine (LNMED), Institute of Technology and Research (ITP), Av. Murilo Dantas 300, Aracaju 49010-390, Brazil
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Industrial Biotechnology Program, University of Tiradentes (UNIT), Av. Murilo Dantas 300, Aracaju 49032-490, Brazil
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Tiradentes Institute, 150 Mt Vernon St, Dorchester, MA 02125, USA
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Laboratory of Nanotechnology and Nanomedicine, Institute of Technology and Research, Aracaju SE 49032-490, Brazil
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School of Pharmacy, University Tiradentes, Aracaju SE 49032-490, Brazil
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School of Biology and Environment, University of Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, P-5001-801 Vila Real, Portugal
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Centre for Research and Technology of Agro-Environmental and Biological Sciences (CITAB), University of Trás-os-Montes e Alto Douro (UTAD), P-5001-801 Vila Real, Portugal
*
Authors to whom correspondence should be addressed.
Received: 20 January 2020 / Revised: 9 February 2020 / Accepted: 13 February 2020 / Published: 16 February 2020
(This article belongs to the Special Issue Nano-Micro Encapsulation of Drugs)
We have developed a new cationic solid lipid nanoparticle (SLN) formulation, composed of Compritol ATO 888, poloxamer 188 and cetyltrimethylammonium bromide (CTAB), to load perillaldehyde 1,2-epoxide, and surface-tailored with a monoclonal antibody for site-specific targeting of human epithelial growth receptor 2 (HER2). Perillaldehyde 1,2-epoxide-loaded cationic SLN (cPa-SLN), with a mean particle size (z-Ave) of 275.31 ± 4.78 nm and polydispersity index (PI) of 0.303 ± 0.081, were produced by high shear homogenization. An encapsulation efficiency of cPa-SLN above 80% was achieved. The release of perillaldehyde 1,2-epoxide from cationic SLN followed the Korsemeyer–Peppas kinetic model, which is typically seen in nanoparticle formulations. The lipid peroxidation of cPa-SLN was assessed by the capacity to produce thiobarbituric acid-reactive substances, while the antioxidant activity was determined by the capacity to scavenge the stable radical DPPH. The surface functionalization of cPa-SLN with the antibody was done via streptavidin-biotin interaction, monitoring z-Ave, PI and ZP of the obtained assembly (cPa-SLN-SAb), as well as its stability in phosphate buffer. The effect of plain cationic SLN (c-SLN, monoterpene free), cPa-SLN and cPa-SLN-SAb onto the MCF-7 cell lines was evaluated in a concentration range from 0.01 to 0.1 mg/mL, confirming that streptavidin adsorption onto cPa-SLN-SAb improved the cell viability in comparison to the cationic cPa-SLN. View Full-Text
Keywords: perillaldehyde 1,2-epoxide; Compritol ATO 888; cationic SLN; streptavidin adsorption; MCF-7 cells perillaldehyde 1,2-epoxide; Compritol ATO 888; cationic SLN; streptavidin adsorption; MCF-7 cells
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MDPI and ACS Style

Souto, E.B.; Souto, S.B.; Zielinska, A.; Durazzo, A.; Lucarini, M.; Santini, A.; Horbańczuk, O.K.; Atanasov, A.G.; Marques, C.; Andrade, L.N.; Silva, A.M.; Severino, P. Perillaldehyde 1,2-epoxide Loaded SLN-Tailored mAb: Production, Physicochemical Characterization and In Vitro Cytotoxicity Profile in MCF-7 Cell Lines. Pharmaceutics 2020, 12, 161. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics12020161

AMA Style

Souto EB, Souto SB, Zielinska A, Durazzo A, Lucarini M, Santini A, Horbańczuk OK, Atanasov AG, Marques C, Andrade LN, Silva AM, Severino P. Perillaldehyde 1,2-epoxide Loaded SLN-Tailored mAb: Production, Physicochemical Characterization and In Vitro Cytotoxicity Profile in MCF-7 Cell Lines. Pharmaceutics. 2020; 12(2):161. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics12020161

Chicago/Turabian Style

Souto, Eliana B., Selma B. Souto, Aleksandra Zielinska, Alessandra Durazzo, Massimo Lucarini, Antonello Santini, Olaf K. Horbańczuk, Atanas G. Atanasov, Conrado Marques, Luciana N. Andrade, Amélia M. Silva, and Patricia Severino. 2020. "Perillaldehyde 1,2-epoxide Loaded SLN-Tailored mAb: Production, Physicochemical Characterization and In Vitro Cytotoxicity Profile in MCF-7 Cell Lines" Pharmaceutics 12, no. 2: 161. https://0-doi-org.brum.beds.ac.uk/10.3390/pharmaceutics12020161

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