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Interaction of a Short Peptide with G-Quadruplex-Forming Sequences: An SRCD and CD Study

Institute of Biomolecular Chemistry of CNR, Via F. Marzolo, 1, 35131 Padova, Italy
Department of Chemical Sciences, University of Padua, Via F. Marzolo, 1, 35131 Padova, Italy
Department of Pharmaceutical and Pharmacological Sciences, University of Padua, Largo Meneghetti, 2, 35131 Padova, Italy
Diamond Light Source Ltd., Harwell Science and Innovation Campus, Didcot OX11 0DE, UK
School of Pharmacy, University of Reading, Reading RG6 6DX, UK
Author to whom correspondence should be addressed.
Academic Editor: Giancarlo Morelli
Received: 10 June 2021 / Revised: 9 July 2021 / Accepted: 13 July 2021 / Published: 21 July 2021
(This article belongs to the Special Issue Pharmaceutical Applications of Bioactive Peptides)
G-quadruplex (G4) forming DNA sequences were recently found to play a crucial role in the regulation of genomic processes such as replication, transcription and translation, also related to serious diseases. Therefore, systems capable of controlling DNA and RNA G-quadruplex structures would be useful for the modulation of various cellular events. In particular, peptides represent good candidates for targeting G-quadruplex structures, since they are easily tailored to enhance their functionality. In this work, we analyzed, by circular dichroism and synchrotron radiation circular dichroism spectroscopies, the interaction of a 25-residue peptide deriving from RHAU helicases (Rhau25) with three G-quadruplex-forming oligonucleotide sequences, in both sodium- and potassium-containing buffers, the most relevant monovalent cations in physiological conditions. The peptide displayed greater affinity for the G4 sequences adopting a parallel structure. However, it showed the ability to also interact with antiparallel or hybrid G-quadruplex structures, inducing a conformation conversion to the parallel structure. The stability of the oligonucleotide structure alone or in presence of the Rhau25 peptide was studied by temperature melting and UV denaturation experiments, and the data showed that the interaction with the peptide stabilized the conformation of oligonucleotide sequences when subjected to stress conditions. View Full-Text
Keywords: G-quadruplex DNA; peptide; photo-stability G-quadruplex DNA; peptide; photo-stability
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MDPI and ACS Style

Honisch, C.; Ragazzi, E.; Hussain, R.; Brazier, J.; Siligardi, G.; Ruzza, P. Interaction of a Short Peptide with G-Quadruplex-Forming Sequences: An SRCD and CD Study. Pharmaceutics 2021, 13, 1104.

AMA Style

Honisch C, Ragazzi E, Hussain R, Brazier J, Siligardi G, Ruzza P. Interaction of a Short Peptide with G-Quadruplex-Forming Sequences: An SRCD and CD Study. Pharmaceutics. 2021; 13(8):1104.

Chicago/Turabian Style

Honisch, Claudia, Eugenio Ragazzi, Rohanah Hussain, John Brazier, Giuliano Siligardi, and Paolo Ruzza. 2021. "Interaction of a Short Peptide with G-Quadruplex-Forming Sequences: An SRCD and CD Study" Pharmaceutics 13, no. 8: 1104.

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