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Brief Report

Dose-Response Relationships in Gene Expression Profiles in a Harbor Seal B Lymphoma Cell Line Exposed to 17α-Ethinyl Estradiol

1
INRS-Institut Armand-Frappier, Laval (QC), Canada
2
Saint-Laurent Centre, Environment Canada, Montreal (QC), Canada
*
Author to whom correspondence should be addressed.
Submission received: 11 March 2017 / Revised: 19 April 2017 / Accepted: 28 April 2017 / Published: 23 May 2017

Abstract

The determination of changes in gene expression profiles with xenobiotic dose will allow identifying biomarkers and modes of toxicant action. The harbor seal (Phoca vitulina) 11B7501 B lymphoma cell line was exposed to 1, 10, 100, 1000, 10,000, or 25,000 μg/L 17α-ethinyl estradiol (EE2, the active compound of the contraceptive pill) for 24 h. Following exposure, RNA was extracted and transformed into cDNA. Transcript expression in exposed vs. control lymphocytes was analyzed via RT-qPCR to identify genes with altered expression. Our analysis indicates that gene expression for all but the reference gene varied with dose, suggesting that different doses induce distinct physiological responses. These findings demonstrate that RT-qPCR could be used to identify immunotoxicity and relative dose in harbor seal leukocytes.
Keywords: Immunotoxicology; Phoca vitulina; RT-qPCR; EE2 Immunotoxicology; Phoca vitulina; RT-qPCR; EE2

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MDPI and ACS Style

Kleinert, C.; Blanchet, M.; Gagné, F.; Fournier, M. Dose-Response Relationships in Gene Expression Profiles in a Harbor Seal B Lymphoma Cell Line Exposed to 17α-Ethinyl Estradiol. J. Xenobiot. 2017, 7, 6702. https://0-doi-org.brum.beds.ac.uk/10.4081/xeno.2017.6702

AMA Style

Kleinert C, Blanchet M, Gagné F, Fournier M. Dose-Response Relationships in Gene Expression Profiles in a Harbor Seal B Lymphoma Cell Line Exposed to 17α-Ethinyl Estradiol. Journal of Xenobiotics. 2017; 7(1):6702. https://0-doi-org.brum.beds.ac.uk/10.4081/xeno.2017.6702

Chicago/Turabian Style

Kleinert, Christine, Matthieu Blanchet, François Gagné, and Michel Fournier. 2017. "Dose-Response Relationships in Gene Expression Profiles in a Harbor Seal B Lymphoma Cell Line Exposed to 17α-Ethinyl Estradiol" Journal of Xenobiotics 7, no. 1: 6702. https://0-doi-org.brum.beds.ac.uk/10.4081/xeno.2017.6702

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