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Sustainable Second-Generation Bioethanol Production from Enzymatically Hydrolyzed Domestic Food Waste Using Pichia anomala as Biocatalyst
Article

On the Optimization of Fermentation Conditions for Enhanced Bioethanol Yields from Starchy Biowaste via Yeast Co-Cultures

1
Department of Biology, College of Science, King Khalid University, Abha 61413, Saudi Arabia
2
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut 71516, Egypt
3
Zografou Campus, School of Chemical Engineering, National Technical University of Athens, GR 15780 Athens, Greece
4
Institute of Chemical Engineering Sciences, Foundation for Research and Technology, GR 26504 Patra, Greece
*
Author to whom correspondence should be addressed.
Academic Editor: David Patiño Vilas
Sustainability 2021, 13(4), 1890; https://0-doi-org.brum.beds.ac.uk/10.3390/su13041890
Received: 15 December 2020 / Revised: 24 January 2021 / Accepted: 1 February 2021 / Published: 9 February 2021
The present study aims to assess the impact of the type of yeast consortium used during bioethanol production from starchy biowastes and to determine the optimal fermentation conditions for enhanced bioethanol production. Three different yeast strains, Saccharomyces cerevisiae, Pichia barkeri, and Candida intermedia were used in mono- and co-cultures with pretreated waste-rice as substrate. The optimization of fermentation conditions i.e., fermentation time, temperature, pH, and inoculum size, was investigated in small-scale batch cultures and subsequently, the optimal conditions were applied for scaling-up and validation of the process in a 7-L fermenter. It was shown that co-culturing of yeasts either in couples or triples significantly enhanced the fermentation efficiency of the process, with ethanol yield reaching 167.80 ± 0.49 g/kg of biowaste during experiments in the fermenter. View Full-Text
Keywords: bioethanol; starchy wastes; hydrolysate; co-cultures; scale-up bioethanol; starchy wastes; hydrolysate; co-cultures; scale-up
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MDPI and ACS Style

Hashem, M.; Alamri, S.A.; Asseri, T.A.Y.; Mostafa, Y.S.; Lyberatos, G.; Ntaikou, I. On the Optimization of Fermentation Conditions for Enhanced Bioethanol Yields from Starchy Biowaste via Yeast Co-Cultures. Sustainability 2021, 13, 1890. https://0-doi-org.brum.beds.ac.uk/10.3390/su13041890

AMA Style

Hashem M, Alamri SA, Asseri TAY, Mostafa YS, Lyberatos G, Ntaikou I. On the Optimization of Fermentation Conditions for Enhanced Bioethanol Yields from Starchy Biowaste via Yeast Co-Cultures. Sustainability. 2021; 13(4):1890. https://0-doi-org.brum.beds.ac.uk/10.3390/su13041890

Chicago/Turabian Style

Hashem, Mohamed, Saad A. Alamri, Tahani A.Y. Asseri, Yasser S. Mostafa, Gerasimos Lyberatos, and Ioanna Ntaikou. 2021. "On the Optimization of Fermentation Conditions for Enhanced Bioethanol Yields from Starchy Biowaste via Yeast Co-Cultures" Sustainability 13, no. 4: 1890. https://0-doi-org.brum.beds.ac.uk/10.3390/su13041890

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