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Article

Cucurbita argyrosperma Seed Extracts Attenuate Angiogenesis in a Corneal Chemical Burn Model

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Laboratorio de Investigacíon de Productos Naturales, Escuela de Ingeniería Química e Industrias Extractivas, Instituto Politécnico Nacional, Unidad Profesional Adolfo López Mateos, Av. Instituto Politécnico Nacional S/N, Ciudad de México 07708, México
2
División de Investigación, Hospital Juárez de México, Av. Instituto Politécnico Nacional 5160, Magdalena de las Salinas, Gustavo A. Madero, Ciudad de México 07760, México
*
Author to whom correspondence should be addressed.
Received: 15 April 2019 / Revised: 7 May 2019 / Accepted: 17 May 2019 / Published: 27 May 2019
(This article belongs to the Special Issue Nutrition and Eye Health)
Severe corneal inflammation produces opacity or even perforation, scarring, and angiogenesis, resulting in blindness. In this study, we used the cornea to examine the effect of new anti-angiogenic chemopreventive agents. We researched the anti-angiogenic effect of two extracts, methanol (Met) and hexane (Hex), from the seed of Cucurbita argyrosperma, on inflamed corneas. The corneas of Wistar rats were alkali-injured and treated intragastrically for seven successive days. We evaluated: opacity score, corneal neovascularization (CNV) area, re-epithelialization percentage, and histological changes. Also, we assessed the inflammatory (cyclooxigenase-2, nuclear factor-kappaB, and interleukin-1β) and angiogenic (vascular endothelial growth factor A, VEGF-A; -receptor 1, VEGFR1; and -receptor 2, VEGFR2) markers. Levels of Cox-2, Il-1β, and Vegf-a mRNA were also determined. After treatment, we observed a reduction in corneal edema, with lower opacity scores and cell infiltration compared to untreated rats. Treatment also accelerated wound healing and decreased the CNV area. The staining of inflammatory and angiogenic factors was significantly decreased and related to a down-expression of Cox-2, Il-1β, and Vegf. These results suggest that intake of C. argyrosperma seed has the potential to attenuate the angiogenesis secondary to inflammation in corneal chemical damage. View Full-Text
Keywords: Cucurbita argyrosperma; corneal chemical burn; angiogenesis; corneal neovascularization (CNV); vascular endothelial growth factor (VEGF); interleukin-1β (IL-1β); cyclooxigenase-2 (COX-2); nuclear factor-kappaB (NF-κB) Cucurbita argyrosperma; corneal chemical burn; angiogenesis; corneal neovascularization (CNV); vascular endothelial growth factor (VEGF); interleukin-1β (IL-1β); cyclooxigenase-2 (COX-2); nuclear factor-kappaB (NF-κB)
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MDPI and ACS Style

Estrella-Mendoza, M.F.; Jiménez-Gómez, F.; López-Ornelas, A.; Pérez-Gutiérrez, R.M.; Flores-Estrada, J. Cucurbita argyrosperma Seed Extracts Attenuate Angiogenesis in a Corneal Chemical Burn Model. Nutrients 2019, 11, 1184. https://0-doi-org.brum.beds.ac.uk/10.3390/nu11051184

AMA Style

Estrella-Mendoza MF, Jiménez-Gómez F, López-Ornelas A, Pérez-Gutiérrez RM, Flores-Estrada J. Cucurbita argyrosperma Seed Extracts Attenuate Angiogenesis in a Corneal Chemical Burn Model. Nutrients. 2019; 11(5):1184. https://0-doi-org.brum.beds.ac.uk/10.3390/nu11051184

Chicago/Turabian Style

Estrella-Mendoza, María F., Francisco Jiménez-Gómez, Adolfo López-Ornelas, Rosa M. Pérez-Gutiérrez, and Javier Flores-Estrada. 2019. "Cucurbita argyrosperma Seed Extracts Attenuate Angiogenesis in a Corneal Chemical Burn Model" Nutrients 11, no. 5: 1184. https://0-doi-org.brum.beds.ac.uk/10.3390/nu11051184

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