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Article

Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells

1
Research Centre, CHU Ste-Justine, Montreal, QC H3T 1C5, Canada
2
Department of Nutrition, Université de Montréal, Montreal, QC H3T 1A8, Canada
*
Author to whom correspondence should be addressed.
Received: 26 March 2020 / Revised: 13 April 2020 / Accepted: 20 April 2020 / Published: 22 April 2020
(This article belongs to the Section Nutrition and Metabolism)
Background. Metabolic Syndrome (MetS), a major worldwide concern for the public health system, refers to a cluster of key metabolic components, and represents a risk factor for diabetes and cardiovascular diseases. As oxidative stress (OxS) and inflammation are the major triggers of insulin sensitivity (IS), a cardinal MetS feature, the principal aim of the present work is to determine whether glycomacropeptide (GMP), a milk-derived bioactive peptide, exerts beneficial effects on their expression. Methods. Fully differentiated intestinal Caco-2/15 cells are used to evaluate the preventive action of 2 mg/mL GMP against OxS and inflammation induced by the mixture iron-ascorbate (Fe/Asc) (200 μM:2 mM). The potency of GMP of decreasing the production of lipoproteins, including chylomicrons (CM), very-low-density lipoproteins (VLDL) and low-density lipoproteins (LDL) is also assessed. Results. The administration of GMP significantly reduces malondialdehyde, a biomarker of lipid peroxidation, and raises superoxide dismutase 2 and glutathione peroxidase via the induction of the nuclear factor erythroid 2–related factor 2, a transcription factor, which orchestrates cellular antioxidant defenses. Similarly, GMP markedly lowers the inflammatory agents tumor necrosis factor-α and cyclooxygenase-2 via abrogation of the nuclear transcription factor-kB. Moreover, GMP-treated cells show a down-regulation of Fe/Asc-induced mitogen activated protein kinase pathway, suggesting greater IS. Finally, GMP decreases the production of CM, VLDL, and LDL. Conclusions. Our results highlight the effectiveness of GMP in attenuating OxS, inflammation and lipoprotein biogenesis, as well as improving IS, the key components of MetS. Further investigation is needed to elucidate the mechanisms mediating the preventive action of GMP. View Full-Text
Keywords: glycomacropeptide; oxidative stress; inflammation; insulin sensitivity; metabolic syndrome; milk-derived bioactive peptide glycomacropeptide; oxidative stress; inflammation; insulin sensitivity; metabolic syndrome; milk-derived bioactive peptide
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MDPI and ACS Style

Foisy-Sauvé, M.; Ahmarani, L.; Delvin, E.; Sané, A.T.; Spahis, S.; Levy, E. Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells. Nutrients 2020, 12, 1175. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12041175

AMA Style

Foisy-Sauvé M, Ahmarani L, Delvin E, Sané AT, Spahis S, Levy E. Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells. Nutrients. 2020; 12(4):1175. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12041175

Chicago/Turabian Style

Foisy-Sauvé, Mathilde, Lena Ahmarani, Edgard Delvin, Alain T. Sané, Schohraya Spahis, and Emile Levy. 2020. "Glycomacropeptide Prevents Iron/Ascorbate-Induced Oxidative Stress, Inflammation and Insulin Sensitivity with an Impact on Lipoprotein Production in Intestinal Caco-2/15 Cells" Nutrients 12, no. 4: 1175. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12041175

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