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Anti-Obesity Effects of Petasites japonicus (Meowi) Ethanol Extract on RAW 264.7 Macrophages and 3T3-L1 Adipocytes and Its Characterization of Polyphenolic Compounds

1
Department of Food Science and Human Nutrition, Jeonbuk National University, 567 Baekje-daero, Jeonju, Jeollabuk-do 54896, Korea
2
Department of Agrofood Resources, Food and Nutrition Division, National Institute of Agricultural Sciences, 166 Nongsaengmyeong-ro Wanju-gun, Jeollabuk-do 55365, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally.
Received: 25 March 2020 / Revised: 20 April 2020 / Accepted: 26 April 2020 / Published: 29 April 2020
(This article belongs to the Section Phytochemicals and Human Health)
Koreans have been consuming Petasites Japonicus (PJ) as food. Although the therapeutic effect of PJ on allergic or inflammatory reactions associated with asthma has been proven, its effect on obesity is unclear. Therefore, the present study was aimed to assess the obesity related anti-inflammatory and anti-adipogenic effects of ethanol extract PJ (EPJ) on the inflammatory response in RAW 264.7 macrophages and on differentiation in 3T3-L1 adipocytes. In addition, the polyphenolic compound was quantitatively characterized from the EPJ using ultra performance liquid chromatography coupled with diode array detector, quadrupole time-of-flight-mass spectrometry (UPLC-DAD-QToF-MS). In RAW 264.7 or 3T3-L1, reduction of nitric oxide (in macrophages) production as well as monocyte chemoattractant protein-1 and tumor necrosis factor-α were observed. Treatment of EPJ in adipocyte differentiation showed an improvement in adiponectin and lipid accumulation and a significant reduction of PPARγ and FABP-4 mRNA expression levels. On the other hand, mRNA expression of UCP-1, PPARα, and ACO increased in the EPJ treated group. In addition, a total of 26 polyphenolic compounds were detected and of which 12 are reported for the first time from PJ. The higher content of diverse polyphenolic compounds presented in EPJ might be responsible for the observed anti-inflammatory and anti-adipogenic effect. These results suggest that PJ is valuable in improving obesity-related inflammatory responses. View Full-Text
Keywords: Petasites japonicus; anti-adipogenic effects; anti-inflammation; anti-lipogenesis; characterization; ployphenolic compounds; flavonoids; UPLC-DAD-QToF-MS Petasites japonicus; anti-adipogenic effects; anti-inflammation; anti-lipogenesis; characterization; ployphenolic compounds; flavonoids; UPLC-DAD-QToF-MS
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MDPI and ACS Style

Ahn, E.M.; Asamenew, G.; Kim, H.W.; Lee, S.H.; Yoo, S.-M.; Cho, S.-M.; Cha, Y.-S.; Kang, M.-S. Anti-Obesity Effects of Petasites japonicus (Meowi) Ethanol Extract on RAW 264.7 Macrophages and 3T3-L1 Adipocytes and Its Characterization of Polyphenolic Compounds. Nutrients 2020, 12, 1261. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12051261

AMA Style

Ahn EM, Asamenew G, Kim HW, Lee SH, Yoo S-M, Cho S-M, Cha Y-S, Kang M-S. Anti-Obesity Effects of Petasites japonicus (Meowi) Ethanol Extract on RAW 264.7 Macrophages and 3T3-L1 Adipocytes and Its Characterization of Polyphenolic Compounds. Nutrients. 2020; 12(5):1261. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12051261

Chicago/Turabian Style

Ahn, Eun M., Gelila Asamenew, Heon W. Kim, Sang H. Lee, Seon-Mi Yoo, Soo-Muk Cho, Youn-Soo Cha, and Min-Sook Kang. 2020. "Anti-Obesity Effects of Petasites japonicus (Meowi) Ethanol Extract on RAW 264.7 Macrophages and 3T3-L1 Adipocytes and Its Characterization of Polyphenolic Compounds" Nutrients 12, no. 5: 1261. https://0-doi-org.brum.beds.ac.uk/10.3390/nu12051261

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