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In Vitro Non-Genomic Effects of Calcifediol on Human Preosteoblastic Cells

Department of Experimental and Clinical Biomedical Sciences, University of Florence, Viale Pieraccini 6, 50139 Florence, Italy
Fondazione Italiana Ricerca Sulle Malattie dell’Osso (FIRMO Onlus), 50141 Florence, Italy
Bone Metabolic Diseases Unit, Department of Experimental and Clinical Biomedical, Sciences, University of Florence, AOU Careggi, 50139 Florence, Italy
Author to whom correspondence should be addressed.
Academic Editor: Lynnette Ferguson
Received: 14 October 2021 / Revised: 20 November 2021 / Accepted: 24 November 2021 / Published: 25 November 2021
(This article belongs to the Special Issue Vitamin D, Diet and Musculoskeletal Health)
Several recent studies have demonstrated that the direct precursor of vitamin D3, the calcifediol [25(OH)D3], through the binding to the nuclear vitamin D receptor (VDR), is able to regulate the expression of many genes involved in several cellular processes. Considering that itself may function as a VDR ligand, although with a lower affinity, respect than the active form of vitamin D, we have assumed that 25(OH)D3 by binding the VDR could have a vitamin’s D3 activity such as activating non-genomic pathways, and in particular we selected mesenchymal stem cells derived from human adipose tissue (hADMSCs) for the in vitro assessment of the intracellular Ca2+ mobilization in response to 25(OH)D3. Our result reveals the ability of 25(OH)D3 to activate rapid, non-genomic pathways, such as an increase of intracellular Ca2+ levels, similar to what observed with the biologically active form of vitamin D3. hADMSCs loaded with Fluo-4 AM exhibited a rapid and sustained increase in intracellular Ca2+ concentration as a result of exposure to 10−5 M of 25(OH)D3. In this work, we show for the first time the in vitro ability of 25(OH)D3 to induce a rapid increase of intracellular Ca2+ levels in hADMSCs. These findings represent an important step to better understand the non-genomic effects of vitamin D3 and its role in endocrine system. View Full-Text
Keywords: vitamin D3; calcitriol; calcifediol; non-genomic effects; intracellular Ca2+ vitamin D3; calcitriol; calcifediol; non-genomic effects; intracellular Ca2+
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MDPI and ACS Style

Donati, S.; Palmini, G.; Romagnoli, C.; Aurilia, C.; Miglietta, F.; Falsetti, I.; Marini, F.; Zonefrati, R.; Galli, G.; Marcucci, G.; Iantomasi, T.; Brandi, M.L. In Vitro Non-Genomic Effects of Calcifediol on Human Preosteoblastic Cells. Nutrients 2021, 13, 4227.

AMA Style

Donati S, Palmini G, Romagnoli C, Aurilia C, Miglietta F, Falsetti I, Marini F, Zonefrati R, Galli G, Marcucci G, Iantomasi T, Brandi ML. In Vitro Non-Genomic Effects of Calcifediol on Human Preosteoblastic Cells. Nutrients. 2021; 13(12):4227.

Chicago/Turabian Style

Donati, Simone, Gaia Palmini, Cecilia Romagnoli, Cinzia Aurilia, Francesca Miglietta, Irene Falsetti, Francesca Marini, Roberto Zonefrati, Gianna Galli, Gemma Marcucci, Teresa Iantomasi, and Maria L. Brandi. 2021. "In Vitro Non-Genomic Effects of Calcifediol on Human Preosteoblastic Cells" Nutrients 13, no. 12: 4227.

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