Next Article in Journal
Exercise Effects on Bone Mineral Density in Men
Next Article in Special Issue
Excess Vitamins or Imbalance of Folic Acid and Choline in the Gestational Diet Alter the Gut Microbiota and Obesogenic Effects in Wistar Rat Offspring
Previous Article in Journal
Effects of Simultaneous Exposure to a Western Diet and Wheel-Running Training on Brain Energy Metabolism in Female Rats
Previous Article in Special Issue
Association of Nutrition in Early Childhood with Body Composition and Leptin in Later Childhood and Early Adulthood
Article

DHA Supplementation of Obese Rats throughout Pregnancy and Lactation Modifies Milk Composition and Anxiety Behavior of Offspring

1
Reproductive Biology Department, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14080, Mexico
2
CONACyT-Cátedras, Reproductive Biology Department, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14080, Mexico
3
Department of Animal Science, University of Wyoming, Laramie, WY 82071, USA
4
Department of Animal Nutrition, Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Mexico City 14080, Mexico
5
Center for Health Systems Research, Instituto Nacional de Salud Pública, Cuernavaca 62100, Mexico
6
Laboratory of Pharmacology and Toxicology, Hospital Infantil de México Federico Gómez, Mexico City 06720, Mexico
7
INRAE, UMR1280 Physiologie des Adaptations Nutritionnelles, Université de Nantes, Nantes Atlantique Université, 44096 Nantes, France
*
Author to whom correspondence should be addressed.
Academic Editors: Valerie Verhasselt, Lieke van den Elsen and Lindsay Brown
Received: 24 August 2021 / Revised: 18 November 2021 / Accepted: 20 November 2021 / Published: 26 November 2021
(This article belongs to the Special Issue Early-Life Nutrition and Metabolic Disorders in Later Life)
We investigated if supplementing obese mothers (MO) with docosahexaenoic acid (DHA) improves milk long-chain polyunsaturated fatty acid (LCPUFA) composition and offspring anxiety behavior. From weaning throughout pregnancy and lactation, female Wistar rats ate chow (C) or a high-fat diet (MO). One month before mating and through lactation, half the mothers received 400 mg DHA kg−1 d−1 orally (C+DHA or MO+DHA). Offspring ate C after weaning. Maternal weight, total body fat, milk hormones, and milk nutrient composition were determined. Pups’ milk nutrient intake was evaluated, and behavioral anxiety tests were conducted. MO exhibited increased weight and total fat, and higher milk corticosterone, leptin, linoleic, and arachidonic acid (AA) concentrations, and less DHA content. MO male and female offspring had higher ω-6/ ω-3 milk consumption ratios. In the elevated plus maze, female but not male MO offspring exhibited more anxiety. MO+DHA mothers exhibited lower weight, total fat, milk leptin, and AA concentrations, and enhanced milk DHA. MO+DHA offspring had a lower ω-6/ω-3 milk intake ratio and reduced anxiety vs. MO. DHA content was greater in C+DHA milk vs. C. Supplementing MO mothers with DHA improves milk composition, especially LCPUFA content and ω-6/ω-3 ratio reducing offspring anxiety in a sex-dependent manner. View Full-Text
Keywords: pregnancy; obesity; breastfeeding; behavioral disorders; LCPUFA; interventions pregnancy; obesity; breastfeeding; behavioral disorders; LCPUFA; interventions
Show Figures

Figure 1

MDPI and ACS Style

Zambrano, E.; Rodríguez-González, G.L.; Reyes-Castro, L.A.; Bautista, C.J.; Castro-Rodríguez, D.C.; Juárez-Pilares, G.; Ibáñez, C.A.; Hernández-Rojas, A.; Nathanielsz, P.W.; Montaño, S.; Arredondo, A.; Huang, F.; Bolaños-Jiménez, F. DHA Supplementation of Obese Rats throughout Pregnancy and Lactation Modifies Milk Composition and Anxiety Behavior of Offspring. Nutrients 2021, 13, 4243. https://0-doi-org.brum.beds.ac.uk/10.3390/nu13124243

AMA Style

Zambrano E, Rodríguez-González GL, Reyes-Castro LA, Bautista CJ, Castro-Rodríguez DC, Juárez-Pilares G, Ibáñez CA, Hernández-Rojas A, Nathanielsz PW, Montaño S, Arredondo A, Huang F, Bolaños-Jiménez F. DHA Supplementation of Obese Rats throughout Pregnancy and Lactation Modifies Milk Composition and Anxiety Behavior of Offspring. Nutrients. 2021; 13(12):4243. https://0-doi-org.brum.beds.ac.uk/10.3390/nu13124243

Chicago/Turabian Style

Zambrano, Elena, Guadalupe L. Rodríguez-González, Luis A. Reyes-Castro, Claudia J. Bautista, Diana C. Castro-Rodríguez, Gimena Juárez-Pilares, Carlos A. Ibáñez, Alejandra Hernández-Rojas, Peter W. Nathanielsz, Sara Montaño, Armando Arredondo, Fengyang Huang, and Francisco Bolaños-Jiménez. 2021. "DHA Supplementation of Obese Rats throughout Pregnancy and Lactation Modifies Milk Composition and Anxiety Behavior of Offspring" Nutrients 13, no. 12: 4243. https://0-doi-org.brum.beds.ac.uk/10.3390/nu13124243

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop