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Article

Assessment of Fusarium Infection and Mycotoxin Contamination of Wheat Kernels and Flour Using Hyperspectral Imaging

1
Institute of Crop Science and Resource Conservation (INRES), Plant Diseases and Plant Protection, University of Bonn, Nussallee 9, 53115 Bonn, Germany
2
Molecular Phytopathology and Mycotoxin Research, University of Goettingen, Grisebachstraße 6, 37077 Goettingen, Germany
3
Institute of Sugar Beet Research (IfZ), Holtenser Landstraße 77, 37079 Goettingen, Germany
*
Author to whom correspondence should be addressed.
Professor Heinz-Wilhelm Dehne passed away during the preparation of this manuscript.
Received: 1 September 2019 / Revised: 18 September 2019 / Accepted: 19 September 2019 / Published: 21 September 2019
(This article belongs to the Section Mycotoxins)
Fusarium head blight (FHB) epidemics in wheat and contamination with Fusarium mycotoxins has become an increasing problem over the last decades. This prompted the need for non-invasive and non-destructive techniques to screen cereal grains for Fusarium infection, which is usually accompanied by mycotoxin contamination. This study tested the potential of hyperspectral imaging to monitor the infection of wheat kernels and flour with three Fusarium species. Kernels of two wheat varieties inoculated at anthesis with F. graminearum, F. culmorum, and F. poae were investigated. Hyperspectral images of kernels and flour were taken in the visible-near infrared (VIS-NIR) (400–1000 nm) and short-wave infrared (SWIR) (1000–2500 nm) ranges. The fungal DNA and mycotoxin contents were quantified. Spectral reflectance of Fusarium-damaged kernels (FDK) was significantly higher than non-inoculated ones. In contrast, spectral reflectance of flour from non-inoculated kernels was higher than that of FDK in the VIS and lower in the NIR and SWIR ranges. Spectral reflectance of kernels was positively correlated with fungal DNA and deoxynivalenol (DON) contents. In the case of the flour, this correlation exceeded r = −0.80 in the VIS range. Remarkable peaks of correlation appeared at 1193, 1231, 1446 to 1465, and 1742 to 2500 nm in the SWIR range. View Full-Text
Keywords: Triticum aestivum; wheat scab; Fusarium graminearum; Fusarium culmorum; Fusarium poae; non-invasive sensors; fungal DNA; mycotoxins; qPCR; LC-MS/MS Triticum aestivum; wheat scab; Fusarium graminearum; Fusarium culmorum; Fusarium poae; non-invasive sensors; fungal DNA; mycotoxins; qPCR; LC-MS/MS
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MDPI and ACS Style

Alisaac, E.; Behmann, J.; Rathgeb, A.; Karlovsky, P.; Dehne, H.-W.; Mahlein, A.-K. Assessment of Fusarium Infection and Mycotoxin Contamination of Wheat Kernels and Flour Using Hyperspectral Imaging. Toxins 2019, 11, 556. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11100556

AMA Style

Alisaac E, Behmann J, Rathgeb A, Karlovsky P, Dehne H-W, Mahlein A-K. Assessment of Fusarium Infection and Mycotoxin Contamination of Wheat Kernels and Flour Using Hyperspectral Imaging. Toxins. 2019; 11(10):556. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11100556

Chicago/Turabian Style

Alisaac, Elias, Jan Behmann, Anna Rathgeb, Petr Karlovsky, Heinz-Wilhelm Dehne, and Anne-Katrin Mahlein. 2019. "Assessment of Fusarium Infection and Mycotoxin Contamination of Wheat Kernels and Flour Using Hyperspectral Imaging" Toxins 11, no. 10: 556. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11100556

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