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Article

Treatment and Prevention of Recurrent Clostridium difficile Infection with Functionalized Bovine Antibody-Enriched Whey in a Hamster Primary Infection Model

1
Chair of Food and Bioprocess Engineering, Technical University of Munich, 85354 Freising, Germany
2
ZIEL Institute for Food & Health, Technical University of Munich, 85354 Freising, Germany
3
University of North Texas Health Science Center, Fort Worth, TX 76107-2699, USA
4
tgcBIOMICS GmbH, 55411 Bingen, Germany
5
Chair of Animal Physiology and Immunology, Technical University of Munich, 85354 Freising, Germany
6
Biosys UK Limited, London, SW1H, 9BP, UK
7
School of Life Science, Technical University of Munich, 85354 Freising, Germany
*
Author to whom correspondence should be addressed.
Received: 19 December 2018 / Revised: 29 January 2019 / Accepted: 1 February 2019 / Published: 6 February 2019
Toxin-induced Clostridium difficile infection (CDI) is a major disease characterized by severe diarrhea and high morbidity rates. The aim with this study was to develop an alternative drug for the treatment of CDI. Cows were repeatedly immunized to establish specific immunoglobulin G and A titers against toxins A (TcdA) and B (TcdB) and against C. difficile cells in mature milk or colostrum. The effect of three different concentrations of anti-C. difficile whey protein isolates (anti-CD-WPI) and the standard of care antibiotic vancomycin were investigated in an animal model of CD infected hamsters (6 groups, with 10 hamsters each). WPI obtained from the milk of exactly the same cows pre-immunization and a vehicle group served as negative controls. The survival of hamsters receiving anti-CD-WPI was 50, 80 and 100% compared to 10 and 0% for the control groups, respectively. Vancomycin suppressed the growth of C. difficile and thus protected the hamsters at the time of administration, but 90% of these hamsters nevertheless died shortly after discontinuation of treatment. In contrast, the surviving hamsters of the anti-CD-WPI groups survived the entire study period, although they were treated for only 75 h. The specific antibodies not only inactivated the toxins for initial suppression of CDI, but also provoked the inhibition of C. difficile growth after discontinuation, thus preventing recurrence. Oral administration of anti-CD-WPI is a functional therapy of CDI in infected hamsters for both primary treatment and prevention of recurrence. Thus, anti-CD-WPI could address the urgent unmet medical need for treating and preventing recurrent CDI in humans. View Full-Text
Keywords: Clostridium difficile infection; sIgA; bovine milk; prevention of recurrence; hamster model of CDI Clostridium difficile infection; sIgA; bovine milk; prevention of recurrence; hamster model of CDI
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MDPI and ACS Style

Heidebrecht, H.-J.; Weiss, W.J.; Pulse, M.; Lange, A.; Gisch, K.; Kliem, H.; Mann, S.; Pfaffl, M.W.; Kulozik, U.; von Eichel-Streiber, C. Treatment and Prevention of Recurrent Clostridium difficile Infection with Functionalized Bovine Antibody-Enriched Whey in a Hamster Primary Infection Model. Toxins 2019, 11, 98. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11020098

AMA Style

Heidebrecht H-J, Weiss WJ, Pulse M, Lange A, Gisch K, Kliem H, Mann S, Pfaffl MW, Kulozik U, von Eichel-Streiber C. Treatment and Prevention of Recurrent Clostridium difficile Infection with Functionalized Bovine Antibody-Enriched Whey in a Hamster Primary Infection Model. Toxins. 2019; 11(2):98. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11020098

Chicago/Turabian Style

Heidebrecht, Hans-Jürgen, William J Weiss, Mark Pulse, Anton Lange, Karina Gisch, Heike Kliem, Sacha Mann, Michael W. Pfaffl, Ulrich Kulozik, and Christoph von Eichel-Streiber. 2019. "Treatment and Prevention of Recurrent Clostridium difficile Infection with Functionalized Bovine Antibody-Enriched Whey in a Hamster Primary Infection Model" Toxins 11, no. 2: 98. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11020098

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