Next Article in Journal
Intracellular Trafficking and Translocation of Pertussis Toxin
Previous Article in Journal
In Situ Collection and Preservation of Intact Microcystis Colonies to Assess Population Diversity and Microcystin Quotas
Article

Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece

1
Department of Botany, School of Biology, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Greece
2
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, GR-541 24 Thessaloniki, Greece
3
Laboratory of Photo-Catalytic Processes and Environmental Chemistry, Institute of Nanoscience & Nanotechnology, National Center for Scientific Research “Demokritos”, Patriarchou Grigoriou & Neapoleos, Agia Paraskevi, 15341 Athens, Greece
*
Author to whom correspondence should be addressed.
Received: 24 June 2019 / Revised: 12 July 2019 / Accepted: 23 July 2019 / Published: 25 July 2019
(This article belongs to the Section Marine and Freshwater Toxins)
Cyanobacteria are a diverse group of photosynthetic Gram-negative bacteria that produce an array of secondary compounds with selective bioactivity against a broad spectrum of organisms and cell lines. In this study, 29 strains isolated from freshwaters in Greece were classified using a polyphasic approach and assigned to Chroococcales, Synechococcales, and Nostocales, representing 11 genera and 17 taxa. There were good agreements between 16S ribosomal RNA (rRNA)–cpcBA–internal genetic spacer (IGS) characterization and morphological features, except for the JaaginemaLimnothrix group which appears intermixed and needs further elucidation. Methanol extracts of the strains were analyzed for cyanotoxin production and tested against pathogenic bacteria species and several cancer cell lines. We report for the first time a Nostoc oryzae strain isolated from rice fields capable of producing microcystins (MCs) and a Chlorogloeopsis fritschii strain isolated from the plankton of a lake, suggesting that this species may also occur in freshwater temperate habitats. Strains with very high or identical 16S rRNA gene sequences displayed different antibacterial and cytotoxic activities. Extracts from Synechococcus cf. nidulans showed the most potent antibacterial activity against Staphylococcus aureus, whereas Jaaginema sp. strains exhibited potent cytotoxic activities against human colorectal adenocarcinoma and hepatocellular carcinoma cells. Jaaginema Thessaloniki Aristotle University Microalgae and Cyanobacteria (TAU-MAC) 0110 and 0210 strains caused pronounced changes in the actin network and triggered the formation of numerous lipid droplets in hepatocellular carcinoma and green monkey kidney cells, suggesting oxidative stress and/or mitochondrial damage leading to apoptosis. View Full-Text
Keywords: polyphasic taxonomy; antibacterial; cytotoxicity; microcystins; Jaaginema; Synechococcus polyphasic taxonomy; antibacterial; cytotoxicity; microcystins; Jaaginema; Synechococcus
Show Figures

Figure 1

MDPI and ACS Style

Gkelis, S.; Panou, M.; Konstantinou, D.; Apostolidis, P.; Kasampali, A.; Papadimitriou, S.; Kati, D.; Di Lorenzo, G.M.; Ioakeim, S.; Zervou, S.-K.; Christophoridis, C.; Triantis, T.M.; Kaloudis, T.; Hiskia, A.; Arsenakis, M. Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins 2019, 11, 436. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11080436

AMA Style

Gkelis S, Panou M, Konstantinou D, Apostolidis P, Kasampali A, Papadimitriou S, Kati D, Di Lorenzo GM, Ioakeim S, Zervou S-K, Christophoridis C, Triantis TM, Kaloudis T, Hiskia A, Arsenakis M. Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece. Toxins. 2019; 11(8):436. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11080436

Chicago/Turabian Style

Gkelis, Spyros, Manthos Panou, Despoina Konstantinou, Panagiotis Apostolidis, Antonia Kasampali, Sofia Papadimitriou, Dominiki Kati, Giorgia M. Di Lorenzo, Stamatia Ioakeim, Sevasti-Kiriaki Zervou, Christophoros Christophoridis, Theodoros M. Triantis, Triantafyllos Kaloudis, Anastasia Hiskia, and Minas Arsenakis. 2019. "Diversity, Cyanotoxin Production, and Bioactivities of Cyanobacteria Isolated from Freshwaters of Greece" Toxins 11, no. 8: 436. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11080436

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop