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Pink Bollworm Resistance to Bt Toxin Cry1Ac Associated with an Insertion in Cadherin Exon 20
Article

The Cadherin Cry1Ac Binding-Region is Necessary for the Cooperative Effect with ABCC2 Transporter Enhancing Insecticidal Activity of Bacillus thuringiensis Cry1Ac Toxin

1
Institute of Entomology, School of Life Sciences, Central China Normal University, Wuhan 430079, China
2
Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
3
State Key Laboratory for Biology of Plant Disease and Insect Pests, Chinese Academy of Agricultural Sciences, West Yuanmingyuan Road, Beijing 100193, China
4
Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, Cuernavaca 62250, Morelos, Mexico
*
Author to whom correspondence should be addressed.
These authors equally contributed to this work.
Received: 17 August 2019 / Revised: 4 September 2019 / Accepted: 10 September 2019 / Published: 14 September 2019
(This article belongs to the Special Issue Insecticidal Toxins from Bacillus thuringiensis)
Bacillus thuringiensis Cry1Ac toxin binds to midgut proteins, as cadherin (CAD) and ABCC2 transporter, to form pores leading to larval death. In cell lines, co-expression of CAD and ABCC2 enhance Cry1Ac toxicity significantly, but the mechanism remains elusive. Here, we show that the expression of Helicoverpa armigera CAD (HaCAD-GFP) in Hi5 cells induces susceptibility to Cry1Ac and enhanced Cry1Ac toxicity when co-expressed with H. armigera ABCC2 (HaABCC2-GFP), since Cry1Ac toxicity increased 735-fold compared to Hi5 cells expressing HaCAD-GFP alone or 28-fold compared to HaABCC2-GFP alone. In contrast, the expression of the Spodoptera litura CAD (SlCAD-GFP) in Hi5 cells did not induce susceptibility to Cry1Ac nor it potentiated Cry1Ac toxicity with HaABCC2-GFP. To identify the CAD regions involved in the enhancement of Cry1Ac toxicity with ABCC2, the different CAD domains were replaced between SlCAD-GFP and HaCad-GFP proteins, and cytotoxicity assays were performed in Hi5 cells in the absence or presence of HaABCC2-GFP. The HaCAD toxin-binding region (TB), specifically the CAD repeat-11, was necessary to enhance Cry1Ac toxicity with ABCC2. We propose that CAD TB is involved in recruiting Cry1Ac to localize it in a good position for its interaction with the ABCC2, resulting in efficient toxin membrane insertion enhancing Cry1Ac toxicity. View Full-Text
Keywords: Helicoverpa armigera; Spodoptera litura; cadherin; ABCC2 transporter; Bacillus thuringiensis; synergism; Cry1Ac Helicoverpa armigera; Spodoptera litura; cadherin; ABCC2 transporter; Bacillus thuringiensis; synergism; Cry1Ac
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MDPI and ACS Style

Ma, Y.; Zhang, J.; Xiao, Y.; Yang, Y.; Liu, C.; Peng, R.; Yang, Y.; Bravo, A.; Soberón, M.; Liu, K. The Cadherin Cry1Ac Binding-Region is Necessary for the Cooperative Effect with ABCC2 Transporter Enhancing Insecticidal Activity of Bacillus thuringiensis Cry1Ac Toxin. Toxins 2019, 11, 538. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11090538

AMA Style

Ma Y, Zhang J, Xiao Y, Yang Y, Liu C, Peng R, Yang Y, Bravo A, Soberón M, Liu K. The Cadherin Cry1Ac Binding-Region is Necessary for the Cooperative Effect with ABCC2 Transporter Enhancing Insecticidal Activity of Bacillus thuringiensis Cry1Ac Toxin. Toxins. 2019; 11(9):538. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11090538

Chicago/Turabian Style

Ma, Yuemin, Jianfeng Zhang, Yutao Xiao, Yanchao Yang, Chenxi Liu, Rong Peng, Yongbo Yang, Alejandra Bravo, Mario Soberón, and Kaiyu Liu. 2019. "The Cadherin Cry1Ac Binding-Region is Necessary for the Cooperative Effect with ABCC2 Transporter Enhancing Insecticidal Activity of Bacillus thuringiensis Cry1Ac Toxin" Toxins 11, no. 9: 538. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins11090538

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