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Article

High Titer Persistent Neutralizing Antibodies Induced by TSST-1 Variant Vaccine Against Toxic Shock Cytokine Storm

1
Biomedizinische Forschung & Bio-Produkte AG, Lazarettgasse 19, 1090 Vienna, Austria
2
Department of Clinical Pharmacology, Medical University of Vienna, 1090 Vienna, Austria
3
Department of Emergency Medicine, Medical University of Vienna, 1090 Vienna, Austria
*
Author to whom correspondence should be addressed.
Received: 14 August 2020 / Revised: 17 September 2020 / Accepted: 29 September 2020 / Published: 2 October 2020
Staphylococcal superantigen toxins lead to a devastating cytokine storm resulting in shock and multi-organ failure. We have previously assessed the safety and immunogenicity of a recombinant toxic shock syndrome toxin 1 variant vaccine (rTSST-1v) in clinical trials (NCT02971670 and NCT02340338). The current study assessed neutralizing antibody titers after repeated vaccination with escalating doses of rTSST-1v. At study entry, 23 out of 34 subjects (67.6%) had neutralizing antibody titers inhibiting T cell activation as determined by 3H-thymidine incorporation at a serum dilution of ≤1:100 with similar figures for inhibition of IL-2 activation (19 of 34 subjects, 55.9%) as assessed by quantitative PCR. After the first vaccination, numbers of subjects with neutralization titers inhibiting T cell activation (61.7% ≥ 1:1000) and inhibiting IL-2 gene induction (88.2% ≥ 1:1000) increased. The immune response was augmented after the second vaccination (inhibiting T cell activation: 78.8% ≥ 1:1000; inhibiting IL-2 induction: 93.9% ≥ 1:1000) corroborated with a third immunization months later in a small subgroup of subjects. Assessment of IFNγ, TNFα and IL-6 inhibition revealed similar results, whereas neutralization titers did not change in placebo participants. Antibody titer studies show that vaccination with rTSST-1v in subjects with no/low neutralizing antibodies can rapidly induce high titer neutralizing antibodies persisting over months. View Full-Text
Keywords: Staphylococcus; toxic shock syndrome; neutralizing antibodies; immunogenicity Staphylococcus; toxic shock syndrome; neutralizing antibodies; immunogenicity
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MDPI and ACS Style

Roetzer, A.; Stich, N.; Model, N.; Schwameis, M.; Firbas, C.; Jilma, B.; Eibl, M.M. High Titer Persistent Neutralizing Antibodies Induced by TSST-1 Variant Vaccine Against Toxic Shock Cytokine Storm. Toxins 2020, 12, 640. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12100640

AMA Style

Roetzer A, Stich N, Model N, Schwameis M, Firbas C, Jilma B, Eibl MM. High Titer Persistent Neutralizing Antibodies Induced by TSST-1 Variant Vaccine Against Toxic Shock Cytokine Storm. Toxins. 2020; 12(10):640. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12100640

Chicago/Turabian Style

Roetzer, Andreas, Norbert Stich, Nina Model, Michael Schwameis, Christa Firbas, Bernd Jilma, and Martha M. Eibl 2020. "High Titer Persistent Neutralizing Antibodies Induced by TSST-1 Variant Vaccine Against Toxic Shock Cytokine Storm" Toxins 12, no. 10: 640. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12100640

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