Next Article in Journal
Multiplex Immunoassay Techniques for On-Site Detection of Security Sensitive Toxins
Next Article in Special Issue
Roles of Nutrient Limitation on Western Lake Erie CyanoHAB Toxin Production
Previous Article in Journal
Staphylococcal Panton–Valentine Leucocidin and Gamma Haemolysin Target and Lyse Mature Bone Marrow Leucocytes
Previous Article in Special Issue
Co-Occurrence of Cyanobacteria and Cyanotoxins with Other Environmental Health Hazards: Impacts and Implications
Article

Mass Occurrence of Anatoxin-a- and Dihydroanatoxin-a-Producing Tychonema sp. in Mesotrophic Reservoir Mandichosee (River Lech, Germany) as a Cause of Neurotoxicosis in Dogs

1
Limnological Research Station Iffeldorf, Aquatic Systems Biology Unit, Technical University of Munich, Hofmark 1-3, 82393 Iffeldorf, Germany
2
German Environment Agency, Schichauweg 58, 12307 Berlin, Germany
3
Bavarian Health and Food Safety Authority, Veterinärstraße 2, 85764 Oberschleißheim, Germany
4
Center for Clinical Veterinary Medicine, Institute for Veterinary Pathology, Ludwig-Maximilians-University, Veterinärstraße 13, 80539 Munich, Germany
*
Author to whom correspondence should be addressed.
Received: 28 October 2020 / Revised: 17 November 2020 / Accepted: 17 November 2020 / Published: 20 November 2020
In August 2019, three dogs died after bathing in or drinking from Mandichosee, a mesotrophic reservoir of the River Lech (Germany). The dogs showed symptoms of neurotoxic poisoning and intoxication with cyanotoxins was considered. Surface blooms were not visible at the time of the incidents. Benthic Tychonema sp., a potential anatoxin-a (ATX)-producing cyanobacterium, was detected in mats growing on the banks, as biofilm on macrophytes and later as aggregations floating on the lake surface. The dogs’ pathological examinations showed lung and liver lesions. ATX and dihydroanatoxin-a (dhATX) were detected by LC-MS/MS in the stomachs of two dogs and reached concentrations of 563 and 1207 µg/L, respectively. Anatoxins (sum of ATX and dhATX, ATXs) concentrations in field samples from Mandichosee ranged from 0.1 µg/L in the open water to 68,000 µg/L in samples containing a large amount of mat material. Other (neuro)toxic substances were not found. A molecular approach was used to detect toxin genes by PCR and to reveal the cyanobacterial community composition by sequencing. Upstream of Mandichosee, random samples were taken from other Lech reservoirs, uncovering Tychonema and ATXs at several sampling sites. Similar recent findings emphasize the importance of focusing on the investigation of benthic toxic cyanobacteria and applying appropriate monitoring strategies in the future. View Full-Text
Keywords: cyanobacteria; anatoxin-a; dihydroanatoxin-a; Tychonema; neurotoxicosis; cyanotoxins; macrophytes; benthic; tychoplanktic; reservoir cyanobacteria; anatoxin-a; dihydroanatoxin-a; Tychonema; neurotoxicosis; cyanotoxins; macrophytes; benthic; tychoplanktic; reservoir
Show Figures

Figure 1

MDPI and ACS Style

Bauer, F.; Fastner, J.; Bartha-Dima, B.; Breuer, W.; Falkenau, A.; Mayer, C.; Raeder, U. Mass Occurrence of Anatoxin-a- and Dihydroanatoxin-a-Producing Tychonema sp. in Mesotrophic Reservoir Mandichosee (River Lech, Germany) as a Cause of Neurotoxicosis in Dogs. Toxins 2020, 12, 726. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12110726

AMA Style

Bauer F, Fastner J, Bartha-Dima B, Breuer W, Falkenau A, Mayer C, Raeder U. Mass Occurrence of Anatoxin-a- and Dihydroanatoxin-a-Producing Tychonema sp. in Mesotrophic Reservoir Mandichosee (River Lech, Germany) as a Cause of Neurotoxicosis in Dogs. Toxins. 2020; 12(11):726. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12110726

Chicago/Turabian Style

Bauer, Franziska, Jutta Fastner, Bernadett Bartha-Dima, Wolfram Breuer, Almuth Falkenau, Christian Mayer, and Uta Raeder. 2020. "Mass Occurrence of Anatoxin-a- and Dihydroanatoxin-a-Producing Tychonema sp. in Mesotrophic Reservoir Mandichosee (River Lech, Germany) as a Cause of Neurotoxicosis in Dogs" Toxins 12, no. 11: 726. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12110726

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop