Next Article in Journal
Hypervolemia-Induced Immune Disturbances Do Not Involve IL-1ß but IL-6 and IL-10 Activation in Haemodialysis Patients
Next Article in Special Issue
AFB1 Induced Transcriptional Regulation Related to Apoptosis and Lipid Metabolism in Liver of Chicken
Previous Article in Journal
Inhibitory Effects of a Reengineered Anthrax Toxin on Canine Oral Mucosal Melanomas
Previous Article in Special Issue
Genetic Profiling of Aspergillus Isolates with Varying Aflatoxin Production Potential from Different Maize-Growing Regions of Kenya
Article

A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products

by 1,†, 1,†, 1, 1,2,3, 1,2,3,4,5, 1, 1,2,3,4,5,* and 1,2,3,5
1
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Key Laboratory of Detection for Mycotoxins, Ministry of Agriculture, Wuhan 430062, China
3
National Reference Laboratory for Agricultural Testing (Biotoxin), Wuhan 430062, China
4
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China
5
Laboratory of Risk Assessment for Oilseeds Products, Wuhan, Ministry of Agriculture, Wuhan 430062, China
*
Author to whom correspondence should be addressed.
These authors contribute equally to this work.
Received: 7 February 2020 / Revised: 28 February 2020 / Accepted: 1 March 2020 / Published: 3 March 2020
(This article belongs to the Collection Aflatoxins)
Sensitive and point-of-care detection of small toxic molecules plays a key role in food safety. Aflatoxin, a typical small toxic molecule, can cause serious healthcare and economic issues, thereby promoting the development of sensitive and point-of-care detection. Although ELISA is one of the official detection methods, it cannot fill the gap between sensitivity and point-of-care application because it requires a large-scale microplate reader. To employ portable readers in food safety, Pt-catalysis has attracted increasing attention due to its portability and reliability. In this study, we developed a sensitive point-of-care aflatoxin detection (POCAD) method via a portable handheld barometer. We synthesized and characterized [email protected] and [email protected] conjugated with a second antibody ([email protected]). A competitive immunoassay was established based on the homemade monoclonal antibody against aflatoxins. [email protected] was used to catalyze the production of O2 from H2O2 in a sealed vessel. The pressure of O2 was then recorded by a handheld barometer. The aflatoxin concentration was inversely proportional to the pressure recorded via the barometer reading. After optimization, a limit of detection of 0.03 ng/mL and a linear range from 0.09 to 16.0 ng/mL were achieved. Recovery was recorded as 83.1%–112.0% along with satisfactory results regarding inner- and inter-assay precision (relative standard deviation, RSD < 6.4%). Little cross-reaction was observed. Additionally, the POCAD was validated by high-performance liquid chromatography (HPLC) by using peanut and corn samples. The portable POCAD exhibits strong potential for applications in the on-site detection of small toxic molecules to ensure food safety. View Full-Text
Keywords: aflatoxin; mycotoxin; [email protected]; immunoassay; handheld barometer aflatoxin; mycotoxin; [email protected]; immunoassay; handheld barometer
Show Figures

Graphical abstract

MDPI and ACS Style

Zhang, W.; Wu, W.; Cai, C.; Hu, X.; Li, H.; Bai, Y.; Zhang, Z.; Li, P. A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products. Toxins 2020, 12, 158. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12030158

AMA Style

Zhang W, Wu W, Cai C, Hu X, Li H, Bai Y, Zhang Z, Li P. A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products. Toxins. 2020; 12(3):158. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12030158

Chicago/Turabian Style

Zhang, Weiqi, Wenqin Wu, Chong Cai, Xiaofeng Hu, Hui Li, Yizhen Bai, Zhaowei Zhang, and Peiwu Li. 2020. "A Sensitive, Point-of-Care Detection of Small Molecules Based on a Portable Barometer: Aflatoxins In Agricultural Products" Toxins 12, no. 3: 158. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12030158

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop