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Article

Orally Administered Fumonisins Affect Porcine Red Cell Membrane Sodium Pump Activity and Lipid Profile without Apparent Oxidative Damage

1
“MTA-KE-SzIE Mycotoxins in the Food Chain” Research Group, Hungarian Academy of Sciences-Kaposvár University, 7400 Kaposvár, Hungary
2
Faculty of Agricultural and Environmental Sciences, Kaposvár University, 7400 Kaposvár, Hungary
3
Department of Nutrition, Faculty of Agricultural and Environmental Sciences, Szent István University, 2013 Gödöllő, Hungary
4
Fumizol Ltd., 6725 Szeged, Hungary
*
Author to whom correspondence should be addressed.
Received: 22 April 2020 / Revised: 4 May 2020 / Accepted: 7 May 2020 / Published: 12 May 2020
(This article belongs to the Special Issue Effects of Feedborne Mycotoxins on Animal Health)
Weaned piglets (n = 3 × 6) were fed 0, 15 and 30 mg/kg diet fumonisin (FB1, FB2 and FB3, i.e., FBs, a sphinganine analogue mycotoxin), from the age of 35 days for 21 days, to assess mycotoxin induced, dose-dependent changes in the red cells’ membrane. Ouabain sensitive Na+/K+ ATPase activity was determined from lysed red cell membranes, membrane fatty acid (FA) profile was analysed, as well as antioxidant and lipid peroxidation endpoints. Final body weight was higher in the 30 mg/kg group (vs. control), even besides identical cumulative feed intake. After 3 weeks, there was a difference between control and the 30 mg/kg group in red cell membrane sodium pump activity; this change was dose-dependent (sig.: 0.036; R2 = 0.58). Membrane FA profile was strongly saturated with non-systematic inter-group differences; pooled data provided negative correlation with sodium pump activity (all individual membrane n6 FAs). Intracellular antioxidants (reduced glutathione and glutathione peroxidase) and lipid peroxidation indicators (conj. dienes, trienes and malondialdehyde) were non-responsive. We suppose a ceramide synthesis inhibitor (FB1) effect exerted onto the cell membrane, proven to be toxin dose-dependent and increasing sodium pump activity, with only indirect FA compositional correlations and lack of lipid peroxidation. View Full-Text
Keywords: fumonisins; red blood cell; sodium pump; membrane; fatty acids; oxidative stress; pig fumonisins; red blood cell; sodium pump; membrane; fatty acids; oxidative stress; pig
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MDPI and ACS Style

Szabó, A.; Ali, O.; Lóki, K.; Balogh, K.; Mézes, M.; Bartók, T.; Horváth, L.; Kovács, M. Orally Administered Fumonisins Affect Porcine Red Cell Membrane Sodium Pump Activity and Lipid Profile without Apparent Oxidative Damage. Toxins 2020, 12, 318. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12050318

AMA Style

Szabó A, Ali O, Lóki K, Balogh K, Mézes M, Bartók T, Horváth L, Kovács M. Orally Administered Fumonisins Affect Porcine Red Cell Membrane Sodium Pump Activity and Lipid Profile without Apparent Oxidative Damage. Toxins. 2020; 12(5):318. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12050318

Chicago/Turabian Style

Szabó, András, Omeralfaroug Ali, Katalin Lóki, Krisztián Balogh, Miklós Mézes, Tibor Bartók, Levente Horváth, and Melinda Kovács. 2020. "Orally Administered Fumonisins Affect Porcine Red Cell Membrane Sodium Pump Activity and Lipid Profile without Apparent Oxidative Damage" Toxins 12, no. 5: 318. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12050318

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