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Article

Shedding Light on the Venom Proteomes of the Allergy-Relevant Hymenoptera Polistes dominula (European Paper Wasp) and Vespula spp. (Yellow Jacket)

1
Center of Allergy and Environment (ZAUM), Technical University of Munich, School of Medicine and Helmholtz Center Munich, German Research Center for Environmental Health, 85764 Munich, Germany
2
Department of Infection and Immunity, Luxembourg Institute of Health (LIH), 4354 Esch-Sur-Alzette, Luxembourg
3
Department of Clinical and Molecular Sciences, Polytechnic University of Marche, Ancona and Allergy Unit, Department of Internal Medicine, University Hospital of Ancona, 60126 Ancona, Italy
4
Quantitative Biology Unit, Luxembourg Institute of Health (LIH), 1445 Strassen, Luxembourg
5
Department of Dermatology and Allergy Center, Odense Research Center for Anaphylaxis, University of Southern Denmark, 5000 Odense, Denmark
*
Author to whom correspondence should be addressed.
Received: 16 April 2020 / Revised: 12 May 2020 / Accepted: 12 May 2020 / Published: 14 May 2020
(This article belongs to the Section Animal Venoms)
Allergic reactions to stings of Hymenoptera species can have serious or even fatal consequences. If the identification of the culprit insect is possible, venom-specific immunotherapy effectively cures Hymenoptera venom allergies. Although component-resolved diagnostics has strongly evolved in recent years, the differentiation between allergies to closely related species such as Polistes dominula and Vespula spp. is still challenging. In order to generate the basis for new diagnostic and therapeutic strategies, this study aims at resolving the venom proteomes (venomes) of these species. The venoms of P. dominula and Vespula spp. (V. germanica, V. vulgaris) were analyzed by liquid chromatography-mass spectrometry. Resulting proteins were characterized regarding their function, localization and biochemical properties. The analyses yielded 157 proteins in Vespula spp. and 100 in P. dominula venom; 48 proteins, including annotated allergens, were found in both samples. In addition to a variety of venom trace molecules, new allergen candidates such as icarapin-like protein and phospholipase A2 were identified. This study elucidates the venomes of closely related allergy-eliciting Hymenoptera species. The data indicates that relying on marker allergens to differentiate between P. dominula and Vespula spp. venom allergy is probably insufficient and that strategies using cross-reactive major allergens could be more promising. View Full-Text
Keywords: allergen; Hymenoptera venom allergy; European paper wasp; Polistes dominula venom; venom proteome; Vespula spp. venom; yellow jacket allergen; Hymenoptera venom allergy; European paper wasp; Polistes dominula venom; venom proteome; Vespula spp. venom; yellow jacket
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MDPI and ACS Style

Grosch, J.; Hilger, C.; Bilò, M.B.; Kler, S.; Schiener, M.; Dittmar, G.; Bernardin, F.; Lesur, A.; Ollert, M.; Schmidt-Weber, C.B.; Blank, S. Shedding Light on the Venom Proteomes of the Allergy-Relevant Hymenoptera Polistes dominula (European Paper Wasp) and Vespula spp. (Yellow Jacket). Toxins 2020, 12, 323. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12050323

AMA Style

Grosch J, Hilger C, Bilò MB, Kler S, Schiener M, Dittmar G, Bernardin F, Lesur A, Ollert M, Schmidt-Weber CB, Blank S. Shedding Light on the Venom Proteomes of the Allergy-Relevant Hymenoptera Polistes dominula (European Paper Wasp) and Vespula spp. (Yellow Jacket). Toxins. 2020; 12(5):323. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12050323

Chicago/Turabian Style

Grosch, Johannes, Christiane Hilger, Maria B. Bilò, Stephanie Kler, Maximilian Schiener, Gunnar Dittmar, François Bernardin, Antoine Lesur, Markus Ollert, Carsten B. Schmidt-Weber, and Simon Blank. 2020. "Shedding Light on the Venom Proteomes of the Allergy-Relevant Hymenoptera Polistes dominula (European Paper Wasp) and Vespula spp. (Yellow Jacket)" Toxins 12, no. 5: 323. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12050323

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