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Article

A New Laccase of Lac 2 from the White Rot Fungus Cerrena unicolor 6884 and Lac 2-Mediated Degradation of Aflatoxin B1

by 1,2,3, 2,3, 4, 2,3, 2,3 and 1,2,3,*
1
College of Chemical Engineering, Fuzhou University, Fuzhou 350116, China
2
The Key Laboratory of Marine Enzyme Engineering of Fujian Province, Fuzhou University, Fuzhou 350116, China
3
National Engineering Laboratory for High-efficient Enzyme Expression, Fuzhou 350116, China
4
School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong 999077, China
*
Author to whom correspondence should be addressed.
Received: 22 June 2020 / Revised: 22 July 2020 / Accepted: 24 July 2020 / Published: 27 July 2020
(This article belongs to the Section Mycotoxins)
Aflatoxin B1 (AFB1) is a known toxic human carcinogen and can be detoxified by laccases, which are multicopper oxidases that convert several environmental pollutants and toxins. In this study, a new laccase that could catalyze AFB1 degradation was purified and identified from the white-rot fungus Cerrena unicolor 6884. The laccase was purified using (NH4)2SO4 precipitation and anion exchange chromatography, and then identified as Lac 2 through zymogram and UHPLC-MS/MS based on the Illumina transcriptome analysis of C. unicolor 6884. Six putative laccase protein sequences were obtained via functional annotation. The lac 2 cDNA encoding a full-length protein of 512 amino acids was cloned and sequenced to expand the fungus laccase gene library for AFB1 detoxification. AFB1 degradation by Lac 2 was conducted in vitro at pH 7.0 and 45 °C for 24 h. The half-life of AFB1 degradation catalyzed by Lac 2 was 5.16 h. Acetosyringone (AS), Syrinagaldehyde (SA) and [2,2′ -azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)] (ABTS) at 1 mM concentration seemed to be similar mediators for strongly enhancing AFB1 degradation by Lac 2. The product of AFB1 degradation catalyzed by Lac 2 was traced and identified to be Aflatoxin Q1 (AFQ1) based on mass spectrometry data. These findings are promising for a possible application of Lac 2 as a new aflatoxin oxidase in degrading AFB1 present in food and feeds. View Full-Text
Keywords: laccase; Cerrena unicolor; aflatoxin B1; biodegradation; detoxification; product; transcriptome laccase; Cerrena unicolor; aflatoxin B1; biodegradation; detoxification; product; transcriptome
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MDPI and ACS Style

Zhou, Z.; Li, R.; Ng, T.B.; Lai, Y.; Yang, J.; Ye, X. A New Laccase of Lac 2 from the White Rot Fungus Cerrena unicolor 6884 and Lac 2-Mediated Degradation of Aflatoxin B1. Toxins 2020, 12, 476. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12080476

AMA Style

Zhou Z, Li R, Ng TB, Lai Y, Yang J, Ye X. A New Laccase of Lac 2 from the White Rot Fungus Cerrena unicolor 6884 and Lac 2-Mediated Degradation of Aflatoxin B1. Toxins. 2020; 12(8):476. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12080476

Chicago/Turabian Style

Zhou, Zhimin, Renkuan Li, Tzi B. Ng, Yunyun Lai, Jie Yang, and Xiuyun Ye. 2020. "A New Laccase of Lac 2 from the White Rot Fungus Cerrena unicolor 6884 and Lac 2-Mediated Degradation of Aflatoxin B1" Toxins 12, no. 8: 476. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12080476

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