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Article

Immune Suppression Induced by Gallibacterium anatis GtxA During Interaction with Chicken Macrophage-Like HD11 Cells

Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Stigboejlen 4, DK-1870 Frederiksberg C, Denmark
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Received: 29 July 2020 / Revised: 12 August 2020 / Accepted: 18 August 2020 / Published: 20 August 2020
The RTX toxin GtxA expressed by Gallibacterium anatis biovar haemolytica has been proposed a major virulence factor during disease manifestations in the natural host, the chicken. To better understand the role of GtxA in the pathogenesis of G. anatis, we compared the GtxA expressing wildtype strain with its isogenic ∆gtxA mutant that was unable to express GtxA during exposure to chicken macrophage-like HD11 cells. From adhesion and invasion assays, we showed that GtxA appears to promote adhesion and invasion of HD11 cells. By using quantitative RT-PCR, we also demonstrated that the G. anatis expressing GtxA induced a mainly anti-inflammatory (IL-10) host cell response as opposed to the pro-inflammatory (IL-1β, IL-6 and TNF-α) response induced by the GtxA deletion mutant. Interestingly, these results, at least partly, resemble recent responses observed from spleen tissue of chickens infected with the same two bacterial strains. The effect of the GtxA toxin on the type of cell death was less clear. While GtxA clearly induced cell death, our efforts to characterize whether this was due to primarily necrosis or apoptosis through expression analysis of a broad range of apoptosis genes did not reveal clear answers. View Full-Text
Keywords: macrophage; invasion; Gallibacterium anatis; intracellular survival; ∆gtxA mutant macrophage; invasion; Gallibacterium anatis; intracellular survival; gtxA mutant
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MDPI and ACS Style

Tang, B.; Bojesen, A.M. Immune Suppression Induced by Gallibacterium anatis GtxA During Interaction with Chicken Macrophage-Like HD11 Cells. Toxins 2020, 12, 536. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090536

AMA Style

Tang B, Bojesen AM. Immune Suppression Induced by Gallibacterium anatis GtxA During Interaction with Chicken Macrophage-Like HD11 Cells. Toxins. 2020; 12(9):536. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090536

Chicago/Turabian Style

Tang, Bo, and Anders M. Bojesen 2020. "Immune Suppression Induced by Gallibacterium anatis GtxA During Interaction with Chicken Macrophage-Like HD11 Cells" Toxins 12, no. 9: 536. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090536

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