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Article

A Functional ClpXP Protease is Required for Induction of the Accessory Toxin Genes, tst, sed, and sec

1
Division of Applied Microbiology, Department of Chemistry, Lund University, SE-221 00 Lund, Sweden
2
Department of Veterinary and Animal Sciences, University of Copenhagen, 1870 Frederikberg C, Denmark
*
Author to whom correspondence should be addressed.
Received: 6 August 2020 / Revised: 21 August 2020 / Accepted: 26 August 2020 / Published: 28 August 2020
(This article belongs to the Special Issue Staphylococcus aureus Toxins: Promoter or Handicap during Infection)
Staphylococcal toxic shock syndrome is a potentially lethal illness attributed to superantigens produced by Staphylococcus aureus, in particular toxic shock syndrome toxin 1 (TSST-1), but staphylococcal enterotoxins (SEs) are also implicated. The genes encoding these important toxins are carried on mobile genetic elements, and the regulatory networks controlling expression of these toxins remain relatively unexplored. We show here that the highly conserved ClpXP protease stimulates transcription of tst (TSST-1), sec (SEC), and sed (SED) genes in the prototypical strains, SA564 and RN4282. In the wild-type cells, the post-exponential upregulation of toxin gene transcription was proposed to occur via RNAIII-mediated downregulation of the Rot repressor. Contradictive to this model, we showed that the post-exponential induction of tst, sed, and sec transcription did not occur in cells devoid of ClpXP activity, despite the Rot level being diminished. To identify transcriptional regulators with a changed expression in cells devoid of ClpXP activity, RNA sequencing was performed. The RNAseq analysis revealed a number of global virulence regulators that might act downstream of ClpXP, to control expression of tst and other virulence genes. Collectively, the results extend our understanding of the complex transcriptional regulation of the tst, sed, and sec genes. View Full-Text
Keywords: staphylococcus aureus; TSST-1; staphylococcal enterotoxins; virulence gene regulation; ClpXP; mobile genetic elements; RNA-seq staphylococcus aureus; TSST-1; staphylococcal enterotoxins; virulence gene regulation; ClpXP; mobile genetic elements; RNA-seq
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MDPI and ACS Style

Schelin, J.; Cohn, M.T.; Frisk, B.; Frees, D. A Functional ClpXP Protease is Required for Induction of the Accessory Toxin Genes, tst, sed, and sec. Toxins 2020, 12, 553. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090553

AMA Style

Schelin J, Cohn MT, Frisk B, Frees D. A Functional ClpXP Protease is Required for Induction of the Accessory Toxin Genes, tst, sed, and sec. Toxins. 2020; 12(9):553. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090553

Chicago/Turabian Style

Schelin, Jenny, Marianne T. Cohn, Barbro Frisk, and Dorte Frees. 2020. "A Functional ClpXP Protease is Required for Induction of the Accessory Toxin Genes, tst, sed, and sec" Toxins 12, no. 9: 553. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090553

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