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Article

Development of Anti-Idiotypic Nanobody-Phage Based Immuno-Loop-Mediated Isothermal Amplification Assay for Aflatoxins in Peanuts

1
College of Life Sciences, South-Central University for Nationalities, Wuhan 430074, China
2
College of Chemistry and Material Sciences, South-Central University for Nationalities, Wuhan 430074, China
3
Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
*
Author to whom correspondence should be addressed.
Received: 17 August 2020 / Revised: 28 August 2020 / Accepted: 1 September 2020 / Published: 2 September 2020
Aflatoxin contamination in agricultural products has posed serious health hazards and brought huge economic loss in the food and feed industries. Monitoring aflatoxins in various foods and feeds has become a crucial means to protect public health. This study aimed to report an immuno-loop-mediated isothermal amplification (iLAMP) assay by using an anti-idiotypic nanobody-phage for on-site and rapid detection of aflatoxin in real samples. The iLAMP method was developed on the basis of a competitive immunoassay and LAMP reaction performed in a simple water bath. This method can provide visualized test results: violet color represents positive samples while sky blue represents negative. The visual detection limits of iLAMP for aflatoxin B1, B2, G1, and G2 in peanut samples were 1.6, 1.6, 3.2, and 16 μg/kg, respectively. The developed assay was verified with high performance liquid chromatography (HPLC) for the analysis of aflatoxins in peanuts, which demonstrated that the iLAMP method can be applied to the detection of aflatoxin in real samples. The novel iLAMP assay eliminates the need for aflatoxin conjugates, the antibody labeling process, and special equipment, and offers an alternative to existing methods with advantages of time-saving, cost-effectiveness, and ease-of-use. View Full-Text
Keywords: aflatoxin; LAMP; nanobody; immunoassay aflatoxin; LAMP; nanobody; immunoassay
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MDPI and ACS Style

Lei, J.; Han, X.; Tang, X.; Wang, H.; Zhang, Q. Development of Anti-Idiotypic Nanobody-Phage Based Immuno-Loop-Mediated Isothermal Amplification Assay for Aflatoxins in Peanuts. Toxins 2020, 12, 565. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090565

AMA Style

Lei J, Han X, Tang X, Wang H, Zhang Q. Development of Anti-Idiotypic Nanobody-Phage Based Immuno-Loop-Mediated Isothermal Amplification Assay for Aflatoxins in Peanuts. Toxins. 2020; 12(9):565. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090565

Chicago/Turabian Style

Lei, Jiawen, Xiaole Han, Xiaoqian Tang, Haiying Wang, and Qi Zhang. 2020. "Development of Anti-Idiotypic Nanobody-Phage Based Immuno-Loop-Mediated Isothermal Amplification Assay for Aflatoxins in Peanuts" Toxins 12, no. 9: 565. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090565

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