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Gut-Derived Protein-Bound Uremic Toxins
Communication

Antinociceptive Activity of the Skin Secretion of Phyllomedusa rohdei (Amphibia, Anura)

1
Biological Sciences Department, Universidade Estadual de Santa Cruz, Ilhéus, Bahia 45662-000, Brazil
2
Health Area, Universidade Braz Cubas, Mogi das Cruzes, São Paulo 08773-380, Brazil
3
Department of Psychiatry and Human Behavior, University of Mississippi Medical Center, Jackson, MS 39216, USA
4
Institute of Biology, Universidade Estadual de Campinas, Campinas, São Paulo 13083-970, Brazil
5
Health Sciences Department, Universidade Estadual de Santa Cruz, Ilhéus 45662-000, Bahia, Brazil
*
Author to whom correspondence should be addressed.
Received: 9 January 2020 / Revised: 1 March 2020 / Accepted: 3 March 2020 / Published: 11 September 2020
(This article belongs to the Special Issue Amphibian Toxins and Poisons)
Pain is a distressful experience that can have a major impact on an individual’s quality of life. The need for new and better analgesics has been further intensified in light of the current opioid epidemic. Substances obtained from amphibians have been shown to contain bioactive peptides that exert analgesic effects. The genus Phyllomedusa represents an important source of peptides and bioactive components. The aim of this study was to investigate the antinociceptive effects of the skin secretion of Phyllomedusa rohdei in rodent models of pain. The crude skin extract of P. rohdei was tested in different pain models: acetic acid-induced writhing test (mice), formalin test (rats), Von Frey electronic test for hypernociception induced by PGE2 (rats), and hot plate test (mice). Motor-impairing effects were tested using the rota-rod test. The results showed that the skin extract of P. rohdei exerted antinociceptive effects in all pain models tested. Particularly, the highest dose tested of the skin extract decreased acetic acid-induced writhing by 93%, completely blocked formalin-induced nociception both during the acute and inflammatory phases of the test, PGE2-induced hypernociception by 73% and increased latency to paw withdrawal in the hot plate test by 300%. The effects observed in the hot plate test were reversed by pretreatment with selective µ and κ, but not δ, opioid receptor antagonists, indicating a mechanism of action dependent on µ and κ opioid receptors. The results were not influenced by sedative effects. Further studies remain necessary to reveal the specific compounds involved in the antinociceptive effects of P. rohdei skin extract as a new therapeutic tool in pain management. View Full-Text
Keywords: amphibians; antinociceptive activity; pain; Phyllomedusa rohdei; skin secretion; frog amphibians; antinociceptive activity; pain; Phyllomedusa rohdei; skin secretion; frog
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MDPI and ACS Style

Malpezzi-Marinho, E.L.A.; Zanoni, C.I.S.; Molska, G.R.; Paraventi, C.; Wuo-Silva, R.; Berro, L.F.; Parada, C.A.; Tamura, E.K.; Marinho, E.A.V. Antinociceptive Activity of the Skin Secretion of Phyllomedusa rohdei (Amphibia, Anura). Toxins 2020, 12, 589. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090589

AMA Style

Malpezzi-Marinho ELA, Zanoni CIS, Molska GR, Paraventi C, Wuo-Silva R, Berro LF, Parada CA, Tamura EK, Marinho EAV. Antinociceptive Activity of the Skin Secretion of Phyllomedusa rohdei (Amphibia, Anura). Toxins. 2020; 12(9):589. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090589

Chicago/Turabian Style

Malpezzi-Marinho, Elena L.A., Cristiane I.S. Zanoni, Graziella R. Molska, Camila Paraventi, Raphael Wuo-Silva, Laís F. Berro, Carlos A. Parada, Eduardo K. Tamura, and Eduardo A.V. Marinho 2020. "Antinociceptive Activity of the Skin Secretion of Phyllomedusa rohdei (Amphibia, Anura)" Toxins 12, no. 9: 589. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins12090589

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