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Article

New Insectotoxin from Tibellus Oblongus Spider Venom Presents Novel Adaptation of ICK Fold

1
Department of Molecular Neurobiology, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry RAS, 16/10 Miklukho-Maklay Str., 117997 Moscow, Russia
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Skolkovo Institute of Science and Technology, 30 Bld. 1, Bolshoy Boulevard, 121205 Moscow, Russia
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V.L. Talrose Institute for Energy Problems of Chemical Physics, N.N. Semenov Federal Research Center for Chemical Physics, Russian Academy of Sciences, 38 Leninsky Pr., Bld. 2, 119334 Moscow, Russia
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Department of Molecular and Chemical Physics, Moscow Institute of Physics and Technology (National Research University), 9 Institutsky Per., 141700 Dolgoprudny, Russia
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Moscow Institute of Molecular Medicine, Sechenov First Moscow State Medical University, 8 Bld. 2, Trubetskaya Str., 119991 Moscow, Russia
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Toxicology and Pharmacology, University of Leuven (KU Leuven), Campus Gasthuisberg, O&N2, Herestraat 49, P.O. Box 922, B-3000 Leuven, Belgium
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Department of Biological and Medicinal Physics, Moscow Institute of Physics and Technology (National Research University), 9 Institutsky Per., 141700 Dolgoprudny, Russia
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Department of Structural Biology, Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry RAS, 16/10 Miklukho-Maklay Str., 117997 Moscow, Russia
*
Author to whom correspondence should be addressed.
Received: 18 November 2020 / Revised: 21 December 2020 / Accepted: 30 December 2020 / Published: 4 January 2021
(This article belongs to the Section Animal Venoms)
The Tibellus oblongus spider is an active predator that does not spin webs and remains poorly investigated in terms of venom composition. Here, we present a new toxin, named Tbo-IT2, predicted by cDNA analysis of venom glands transcriptome. The presence of Tbo-IT2 in the venom was confirmed by proteomic analyses using the LC-MS and MS/MS techniques. The distinctive features of Tbo-IT2 are the low similarity of primary structure with known animal toxins and the unusual motif of 10 cysteine residues distribution. Recombinant Tbo-IT2 (rTbo-IT2), produced in E. coli using the thioredoxin fusion protein strategy, was structurally and functionally studied. rTbo-IT2 showed insecticidal activity on larvae of the housefly Musca domestica (LD100 200 μg/g) and no activity on the panel of expressed neuronal receptors and ion channels. The spatial structure of the peptide was determined in a water solution by NMR spectroscopy. The Tbo-IT2 structure is a new example of evolutionary adaptation of a well-known inhibitor cystine knot (ICK) fold to 5 disulfide bonds configuration, which determines additional conformational stability and gives opportunities for insectotoxicity and probably some other interesting features. View Full-Text
Keywords: spider venom; transcriptome; proteome; insectotoxin; ICK fold; NMR structure spider venom; transcriptome; proteome; insectotoxin; ICK fold; NMR structure
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MDPI and ACS Style

Korolkova, Y.; Maleeva, E.; Mikov, A.; Lobas, A.; Solovyeva, E.; Gorshkov, M.; Andreev, Y.; Peigneur, S.; Tytgat, J.; Kornilov, F.; Lushpa, V.; Mineev, K.; Kozlov, S. New Insectotoxin from Tibellus Oblongus Spider Venom Presents Novel Adaptation of ICK Fold. Toxins 2021, 13, 29. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13010029

AMA Style

Korolkova Y, Maleeva E, Mikov A, Lobas A, Solovyeva E, Gorshkov M, Andreev Y, Peigneur S, Tytgat J, Kornilov F, Lushpa V, Mineev K, Kozlov S. New Insectotoxin from Tibellus Oblongus Spider Venom Presents Novel Adaptation of ICK Fold. Toxins. 2021; 13(1):29. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13010029

Chicago/Turabian Style

Korolkova, Yuliya; Maleeva, Ekaterina; Mikov, Alexander; Lobas, Anna; Solovyeva, Elizaveta; Gorshkov, Mikhail; Andreev, Yaroslav; Peigneur, Steve; Tytgat, Jan; Kornilov, Fedor; Lushpa, Vladislav; Mineev, Konstantin; Kozlov, Sergey. 2021. "New Insectotoxin from Tibellus Oblongus Spider Venom Presents Novel Adaptation of ICK Fold" Toxins 13, no. 1: 29. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13010029

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