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α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy

College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
Center for Energy Metabolism and Reproduction, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
Shenzhen Agricultural Genome Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 31 October 2020 / Revised: 4 January 2021 / Accepted: 14 January 2021 / Published: 18 January 2021
(This article belongs to the Special Issue Plant Toxins Affecting Animal Health and Production)
The trophoblast, an embryonic tissue, exerts a crucial role in the processes of implantation and placentation. Toxins in food can cause malfunction of trophoblasts, resulting in apoptosis, oxidative stress, and abnormal angiogenesis. α-solanine, a steroidal glycoalkaloid, has antitumor properties on several cancer cells. However, its effect on human trophoblasts has not been elucidated. In this study, human extravillous trophoblast HTR-8/SVneo cells were exposed to α-solanine. Cellular functions including proliferation, migration, invasion, tube formation, and apoptosis were assessed. To monitor autophagic flux, trophoblasts were transfected with a mCherry-GFP-LC3B vector using lentiviral transduction, and expression of autophagy-related biomarkers including Beclin 1, Atgl3, and microtubule-associated protein 1 light chain-3 (MAP1-LC3) were detected. The results show that application of 20 μM α-solanine or above inhibited the cell viability, migration, invasion, and tube formation of the human trophoblast. Cell cycle was arrested at S and G2/M phases in response to 30 μM α-solanine. α-solanine induced apoptosis of HTR-8/SVneo cells and triggered autophagy by increasing the autophagic gene expression and stimulating the formation of autophagosome and autophagic flux. In conclusion, α-solanine can impair the functions of human trophoblast cells via activation of cell apoptosis and autophagy. View Full-Text
Keywords: α-solanine; trophoblast cell; toxicity; apoptosis; autophagy α-solanine; trophoblast cell; toxicity; apoptosis; autophagy
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MDPI and ACS Style

Chen, Z.; Li, C.; Yuan, A.; Gu, T.; Zhang, F.; Fan, X.; Wu, X.; Xiong, X.; Yang, Q. α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy. Toxins 2021, 13, 67.

AMA Style

Chen Z, Li C, Yuan A, Gu T, Zhang F, Fan X, Wu X, Xiong X, Yang Q. α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy. Toxins. 2021; 13(1):67.

Chicago/Turabian Style

Chen, Zhilong; Li, Chen; Yuan, Anwen; Gu, Ting; Zhang, Feng; Fan, Xiujun; Wu, Xiaosong; Xiong, Xingyao; Yang, Qing. 2021. "α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy" Toxins 13, no. 1: 67.

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