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Article

α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy

1
College of Veterinary Medicine, Hunan Agricultural University, Changsha 410128, China
2
Center for Energy Metabolism and Reproduction, Institute of Biomedicine and Biotechnology, Shenzhen Institute of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
3
Shenzhen Agricultural Genome Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 31 October 2020 / Revised: 4 January 2021 / Accepted: 14 January 2021 / Published: 18 January 2021
(This article belongs to the Special Issue Plant Toxins Affecting Animal Health and Production)
The trophoblast, an embryonic tissue, exerts a crucial role in the processes of implantation and placentation. Toxins in food can cause malfunction of trophoblasts, resulting in apoptosis, oxidative stress, and abnormal angiogenesis. α-solanine, a steroidal glycoalkaloid, has antitumor properties on several cancer cells. However, its effect on human trophoblasts has not been elucidated. In this study, human extravillous trophoblast HTR-8/SVneo cells were exposed to α-solanine. Cellular functions including proliferation, migration, invasion, tube formation, and apoptosis were assessed. To monitor autophagic flux, trophoblasts were transfected with a mCherry-GFP-LC3B vector using lentiviral transduction, and expression of autophagy-related biomarkers including Beclin 1, Atgl3, and microtubule-associated protein 1 light chain-3 (MAP1-LC3) were detected. The results show that application of 20 μM α-solanine or above inhibited the cell viability, migration, invasion, and tube formation of the human trophoblast. Cell cycle was arrested at S and G2/M phases in response to 30 μM α-solanine. α-solanine induced apoptosis of HTR-8/SVneo cells and triggered autophagy by increasing the autophagic gene expression and stimulating the formation of autophagosome and autophagic flux. In conclusion, α-solanine can impair the functions of human trophoblast cells via activation of cell apoptosis and autophagy. View Full-Text
Keywords: α-solanine; trophoblast cell; toxicity; apoptosis; autophagy α-solanine; trophoblast cell; toxicity; apoptosis; autophagy
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MDPI and ACS Style

Chen, Z.; Li, C.; Yuan, A.; Gu, T.; Zhang, F.; Fan, X.; Wu, X.; Xiong, X.; Yang, Q. α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy. Toxins 2021, 13, 67. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13010067

AMA Style

Chen Z, Li C, Yuan A, Gu T, Zhang F, Fan X, Wu X, Xiong X, Yang Q. α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy. Toxins. 2021; 13(1):67. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13010067

Chicago/Turabian Style

Chen, Zhilong; Li, Chen; Yuan, Anwen; Gu, Ting; Zhang, Feng; Fan, Xiujun; Wu, Xiaosong; Xiong, Xingyao; Yang, Qing. 2021. "α-Solanine Causes Cellular Dysfunction of Human Trophoblast Cells via Apoptosis and Autophagy" Toxins 13, no. 1: 67. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13010067

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