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Article

Polydatin Protects Bovine Mammary Epithelial Cells against Zearalenone-Induced Apoptosis by Inhibiting Oxidative Responses and Endoplasmic Reticulum Stress

1
Key Laboratory of Zoonosis Research, Department of Animal Science, College of Animal Sciences, Jilin University, Ministry of Education, Changchun 130062, China
2
Institute of Animal Nutrition, Northeast Agricultural University, Harbin 150030, China
*
Author to whom correspondence should be addressed.
YuRong Fu and Yongcheng Jin should be considered joint first author.
Received: 5 January 2021 / Revised: 31 January 2021 / Accepted: 2 February 2021 / Published: 5 February 2021
Zearalenone (ZEA) is a mycotoxin of the Fusarium genus that can cause endoplasmic reticulum (ER) stress and Apoptosis in bovine mammary epithelial cells (MAC-T). Polydatin (PD), a glycoside purified from Polygonum cuspidatum, has antioxidant properties. This study aimed to explore whether PD can alleviate ZEA-induced damage on bovine mammary epithelial cells (MAC-T). We found that incasing the concentration of ZEA (0, 7.5, 15, 30, 60, 90, 120, and 240 μM) gradually decreased the cell viability. PD treatment alone at 5, 10, and 20 μM did not affect cell viability. Follow-up studies then applied 30 μM of ZEA and 5 μM of PD to treat cells; the results showed that the ZEA + PD treatment group effectively reduced cell oxidative damage compared with the ZEA treatment group. The qPCR analysis showed that ZEA treatment significantly up-regulated the expression of ER stress-related genes, relative to the control. However, adding PD significantly down-regulated the expression of ER stress-related genes. The cell apoptosis detection results showed that, compared with the ZEA treatment group, the ZEA + PD treatment group down-regulated the Bax gene and up-regulated the Bcl-2 gene expressions, which reduced the cell apoptosis rate and Caspase-3 activity. Taken together, these results indicate that PD reduces ZEA-induced apoptosis by inhibiting oxidative damage and ER stress. View Full-Text
Keywords: Apoptosis; bovine mammary epithelial cells; endoplasmic reticulum stress; polydatin; zearalenone Apoptosis; bovine mammary epithelial cells; endoplasmic reticulum stress; polydatin; zearalenone
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MDPI and ACS Style

Fu, Y.; Jin, Y.; Shan, A.; Zhang, J.; Tang, H.; Shen, J.; Zhou, C.; Yu, H.; Fang, H.; Zhao, Y.; Wang, J.; Tian, Y. Polydatin Protects Bovine Mammary Epithelial Cells against Zearalenone-Induced Apoptosis by Inhibiting Oxidative Responses and Endoplasmic Reticulum Stress. Toxins 2021, 13, 121. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13020121

AMA Style

Fu Y, Jin Y, Shan A, Zhang J, Tang H, Shen J, Zhou C, Yu H, Fang H, Zhao Y, Wang J, Tian Y. Polydatin Protects Bovine Mammary Epithelial Cells against Zearalenone-Induced Apoptosis by Inhibiting Oxidative Responses and Endoplasmic Reticulum Stress. Toxins. 2021; 13(2):121. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13020121

Chicago/Turabian Style

Fu, Yurong, Yongcheng Jin, Anshan Shan, Jing Zhang, Hongyu Tang, Jinglin Shen, Changhai Zhou, Hao Yu, Hengtong Fang, Yun Zhao, Junxiong Wang, and Yue Tian. 2021. "Polydatin Protects Bovine Mammary Epithelial Cells against Zearalenone-Induced Apoptosis by Inhibiting Oxidative Responses and Endoplasmic Reticulum Stress" Toxins 13, no. 2: 121. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13020121

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