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Article

Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway

Key Laboratory of Zoonosis Research, Ministry of Education, Department of Animal Science, College of Animal Sciences, Jilin University, Changchun 130062, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 5 February 2021 / Revised: 9 March 2021 / Accepted: 11 March 2021 / Published: 12 March 2021
(This article belongs to the Special Issue Histological Effects of Endotoxins)
Lipopolysaccharide (LPS) is an endotoxin, which may cause immune response and inflammation of bovine mammary glands. Mastitis impairs animal health and results in economic loss. Curcumin (CUR) is a naturally occurring diketone compound, which has attracted widespread attention as a potential anti-inflammatory antioxidant. The purpose of this study is to investigate whether CUR can reduce the damage of bovine mammary epithelial cells (MAC-T) induced by LPS and its underlying molecular mechanism. The MAC-T cell line was treated with different concentrations of LPS and CUR for 24 h. The results showed that CUR rescued the decrease of MAC-T cell viability and cell damage induced by LPS. At the same time, 10 µM CUR and 100 µg/mL LPS were used to treat the cells in the follow-up study. The results showed CUR treatment reduced the accumulation of reactive oxygen species (ROS), the expression of inflammatory cytokines (tumor necrosis factor-a (TNF-α), interleukin-8 (IL-8), IL-6 and IL-1β) and the rate of apoptosis induced by LPS. These effects were associated with the activation of the nuclear factor E2-related factor 2 (NFE2L2)-antioxidant response element (ARE) pathway coupled with inactivation of the nuclear factor-κB (NF-κB) inflammatory and caspase/Bcl2 apoptotic pathways. View Full-Text
Keywords: MAC-T cells; oxidative stress; inflammation; apoptosis; curcumin; LPS MAC-T cells; oxidative stress; inflammation; apoptosis; curcumin; LPS
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MDPI and ACS Style

Li, R.; Fang, H.; Shen, J.; Jin, Y.; Zhao, Y.; Wang, R.; Fu, Y.; Tian, Y.; Yu, H.; Zhang, J. Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway. Toxins 2021, 13, 208. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13030208

AMA Style

Li R, Fang H, Shen J, Jin Y, Zhao Y, Wang R, Fu Y, Tian Y, Yu H, Zhang J. Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway. Toxins. 2021; 13(3):208. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13030208

Chicago/Turabian Style

Li, Ruihua, Hengtong Fang, Jinglin Shen, Yongcheng Jin, Yun Zhao, Rui Wang, Yurong Fu, Yue Tian, Hao Yu, and Jing Zhang. 2021. "Curcumin Alleviates LPS-Induced Oxidative Stress, Inflammation and Apoptosis in Bovine Mammary Epithelial Cells via the NFE2L2 Signaling Pathway" Toxins 13, no. 3: 208. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13030208

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