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Study Protocol

Correlations between Low Doses of Zearalenone, Its Carryover Factor and Estrogen Receptor Expression in Different Segments of the Intestines in Pre-Pubertal Gilts—A Study Protocol

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Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
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Department of Environmental Biotechnology, Faculty of Environmental Sciences and Fisheries, University of Warmia and Mazury in Olsztyn, Słoneczna 45G, 10-719 Olsztyn, Poland
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Dairy Industry Innovation Institute Ltd., Kormoranów 1, 11-700 Mrągowo, Poland
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Department of Epizootiology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13/01, 10-718 Olsztyn, Poland
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Department of Human Nutrition, Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Słoneczna 45F, 10-719 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Received: 7 April 2021 / Revised: 14 May 2021 / Accepted: 25 May 2021 / Published: 26 May 2021
(This article belongs to the Special Issue Influence of Deoxynivalenol and Zearalenone in Feed on Animal Health)
Plant materials can be contaminated with Fusarium mycotoxins and their derivatives, whose toxic effects on humans and animals may remain subclinical. Zearalenone (ZEN), a low-molecular-weight compound, is produced by molds in crop plants as a secondary metabolite. The objective of this study will be to analyze the in vivo correlations between very low monotonic doses of ZEN (5, 10, and 15 μg ZEN/kg body weight—BW for 42 days) and the carryover of this mycotoxin and its selected metabolites from the intestinal contents to the intestinal walls, the mRNA expression of estrogen receptor alfa (ERα) and estrogen receptor beta (ERβ) genes, and the mRNA expression of genes modulating selected colon enzymes (CYP1A1 and GSTP1) in the intestinal mucosa of pre-pubertal gilts. An in vivo experiment will be performed on 60 clinically healthy animals with initial BW of 14.5 ± 2 kg. The gilts will be randomly divided into a control group (group C, n = 15) and three experimental groups (group ZEN5, group ZEN10, and group ZEN15; n = 15). Group ZEN5 will be administered per os 5 μg ZEN/kg BW (MABEL), group ZEN10—10 μg ZEN/kg BW (NOAEL), and group ZEN15—15 µg ZEN/kg BW (low LOAEL). In each group, five animals will be euthanized on analytical dates 1 (exposure day 7), 2 (exposure day 21), and 3 (exposure day 42). Samples for in vitro analyses will be collected from an intestinal segment resected from the following regions: the third (horizontal) part of the duodenum, jejunum, ileum, cecum, ascending colon, transverse colon, and descending colon. The experimental material will be collected under special conditions, and it will be transported to specialist laboratories where samples will be obtained for further analyses. View Full-Text
Keywords: zearalenone; digestive tract; carryover factor; ERs mRNA; CYP1A1 mRNA; GSTP1 mRNA; pre-pubertal gilts zearalenone; digestive tract; carryover factor; ERs mRNA; CYP1A1 mRNA; GSTP1 mRNA; pre-pubertal gilts
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MDPI and ACS Style

Gajęcka, M.; Mróz, M.; Brzuzan, P.; Onyszek, E.; Zielonka, Ł.; Lipczyńska-Ilczuk, K.; Przybyłowicz, K.E.; Babuchowski, A.; Gajęcki, M.T. Correlations between Low Doses of Zearalenone, Its Carryover Factor and Estrogen Receptor Expression in Different Segments of the Intestines in Pre-Pubertal Gilts—A Study Protocol. Toxins 2021, 13, 379. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13060379

AMA Style

Gajęcka M, Mróz M, Brzuzan P, Onyszek E, Zielonka Ł, Lipczyńska-Ilczuk K, Przybyłowicz KE, Babuchowski A, Gajęcki MT. Correlations between Low Doses of Zearalenone, Its Carryover Factor and Estrogen Receptor Expression in Different Segments of the Intestines in Pre-Pubertal Gilts—A Study Protocol. Toxins. 2021; 13(6):379. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13060379

Chicago/Turabian Style

Gajęcka, Magdalena, Magdalena Mróz, Paweł Brzuzan, Ewa Onyszek, Łukasz Zielonka, Karolina Lipczyńska-Ilczuk, Katarzyna E. Przybyłowicz, Andrzej Babuchowski, and Maciej T. Gajęcki 2021. "Correlations between Low Doses of Zearalenone, Its Carryover Factor and Estrogen Receptor Expression in Different Segments of the Intestines in Pre-Pubertal Gilts—A Study Protocol" Toxins 13, no. 6: 379. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13060379

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