Next Article in Journal
Botulinum Toxin Injection for Medically Refractory Neurogenic Bladder in Children: A Systematic Review
Next Article in Special Issue
Biosensors for Deoxynivalenol and Zearalenone Determination in Feed Quality Control
Previous Article in Journal
Toxins and Other Bioactive Metabolites in Deep Chlorophyll Layers Containing the Cyanobacteria Planktothrix cf. isothrix in Two Georgian Bay Embayments, Lake Huron
Previous Article in Special Issue
Concentration of Zearalenone, Alpha-Zearalenol and Beta-Zearalenol in the Myocardium and the Results of Isometric Analyses of the Coronary Artery in Prepubertal Gilts
Article

Occurrence of Zearalenone and Its Metabolites in the Blood of High-Yielding Dairy Cows at Selected Collection Sites in Various Disease States

1
Department of Animal Reproduction with Clinic, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
2
Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland
3
Institute of Dairy Industry Innovation Ltd., Kormoranów 1, 11-700 Mrągowo, Poland
4
Department of Human Nutrition, Faculty of Food Sciences, University of Warmia and Mazury in Olsztyn, Słoneczna 45F, 10-719 Olsztyn, Poland
*
Author to whom correspondence should be addressed.
Received: 4 May 2021 / Revised: 24 June 2021 / Accepted: 25 June 2021 / Published: 28 June 2021
(This article belongs to the Special Issue Influence of Deoxynivalenol and Zearalenone in Feed on Animal Health)
Zearalenone (ZEN) and its metabolites, alpha-zearalenol (α-ZEL) and beta-zearalenol (β-ZEL), are ubiquitous in plant materials used as feed components in dairy cattle diets. The aim of this study was to confirm the occurrence of ZEN and its selected metabolites in blood samples collected from different sites in the hepatic portal system (posthepatic–external jugular vein EJV; prehepatic–abdominal subcutaneous vein ASV and median caudal vein MCV) of dairy cows diagnosed with mastitis, ovarian cysts and pyometra. The presence of mycotoxins in the blood plasma was determined with the use of combined separation methods involving immunoaffinity columns, a liquid chromatography system and a mass spectrometry system. The parent compound was detected in all samples collected from diseased cows, whereas α-ZEL and β-ZEL were not identified in any samples, or their concentrations were below the limit of detection (LOD). Zearalenone levels were highest in cows with pyometra, where the percentage share of average ZEN concentrations reached 44%. Blood sampling sites were arranged in the following ascending order based on ZEN concentrations: EJV (10.53 pg/mL, 44.07% of the samples collected from this site), ASV (14.20 pg/mL, 49.59% of the samples) and MCV (26.67 pg/mL, 67.35% of the samples). The results of the study indicate that blood samples for toxicological analyses should be collected from the MCV (prehepatic vessel) of clinically healthy cows and/or cows with subclinical ZEN mycotoxicosis. This sampling site increases the probability of correct diagnosis of subclinical ZEN mycotoxicosis. View Full-Text
Keywords: zearalenone; mastitis; ovarian cysts; pyometra; hepatic portal system; dairy cows zearalenone; mastitis; ovarian cysts; pyometra; hepatic portal system; dairy cows
Show Figures

Figure 1

MDPI and ACS Style

Barański, W.; Gajęcka, M.; Zielonka, Ł.; Mróz, M.; Onyszek, E.; Przybyłowicz, K.E.; Nowicki, A.; Babuchowski, A.; Gajęcki, M.T. Occurrence of Zearalenone and Its Metabolites in the Blood of High-Yielding Dairy Cows at Selected Collection Sites in Various Disease States. Toxins 2021, 13, 446. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070446

AMA Style

Barański W, Gajęcka M, Zielonka Ł, Mróz M, Onyszek E, Przybyłowicz KE, Nowicki A, Babuchowski A, Gajęcki MT. Occurrence of Zearalenone and Its Metabolites in the Blood of High-Yielding Dairy Cows at Selected Collection Sites in Various Disease States. Toxins. 2021; 13(7):446. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070446

Chicago/Turabian Style

Barański, Wojciech, Magdalena Gajęcka, Łukasz Zielonka, Magdalena Mróz, Ewa Onyszek, Katarzyna E. Przybyłowicz, Arkadiusz Nowicki, Andrzej Babuchowski, and Maciej T. Gajęcki 2021. "Occurrence of Zearalenone and Its Metabolites in the Blood of High-Yielding Dairy Cows at Selected Collection Sites in Various Disease States" Toxins 13, no. 7: 446. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070446

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop