Next Article in Journal
Deoxynivalenol Occurrence in Triticale Crops in Romania during the 2012–2014 Period with Extreme Weather Events
Next Article in Special Issue
Effects of Bioinsecticidal Aegerolysin-Based Cytolytic Complexes on Non-Target Organisms
Previous Article in Journal
Paralytic and Amnesic Shellfish Toxins Impacts on Seabirds, Analyses and Management
Previous Article in Special Issue
Ageritin from Pioppino Mushroom: The Prototype of Ribotoxin-Like Proteins, a Novel Family of Specific Ribonucleases in Edible Mushrooms
Article

Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm

1
Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
2
Institute of Anatomy, University of Bern, 3012 Bern, Switzerland
3
Agricultural Institute of Slovenia, 1000 Ljubljana, Slovenia
4
Bioorganic Chemistry Laboratory, Department of Physics, University of Trento, 38123 Trento, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this study.
Received: 8 June 2021 / Revised: 24 June 2021 / Accepted: 25 June 2021 / Published: 29 June 2021
Ostreolysin A6 (OlyA6) is a protein produced by the oyster mushroom (Pleurotus ostreatus). It binds to membrane sphingomyelin/cholesterol domains, and together with its protein partner, pleurotolysin B (PlyB), it forms 13-meric transmembrane pore complexes. Further, OlyA6 binds 1000 times more strongly to the insect-specific membrane sphingolipid, ceramide phosphoethanolamine (CPE). In concert with PlyB, OlyA6 has potent and selective insecticidal activity against the western corn rootworm. We analysed the histological alterations of the midgut wall columnar epithelium of western corn rootworm larvae fed with OlyA6/PlyB, which showed vacuolisation of the cell cytoplasm, swelling of the apical cell surface into the gut lumen, and delamination of the basal lamina underlying the epithelium. Additionally, cryo-electron microscopy was used to explore the membrane interactions of the OlyA6/PlyB complex using lipid vesicles composed of artificial lipids containing CPE, and western corn rootworm brush border membrane vesicles. Multimeric transmembrane pores were formed in both vesicle preparations, similar to those described for sphingomyelin/cholesterol membranes. These results strongly suggest that the molecular mechanism of insecticidal action of OlyA6/PlyB arises from specific interactions of OlyA6 with CPE, and the consequent formation of transmembrane pores in the insect midgut. View Full-Text
Keywords: aegerolysin; bioinsecticide; MACPF-protein; oyster mushroom; pore-forming protein; western corn rootworm aegerolysin; bioinsecticide; MACPF-protein; oyster mushroom; pore-forming protein; western corn rootworm
Show Figures

Graphical abstract

MDPI and ACS Style

Milijaš Jotić, M.; Panevska, A.; Iacovache, I.; Kostanjšek, R.; Mravinec, M.; Skočaj, M.; Zuber, B.; Pavšič, A.; Razinger, J.; Modic, Š.; Trenti, F.; Guella, G.; Sepčić, K. Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm. Toxins 2021, 13, 455. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070455

AMA Style

Milijaš Jotić M, Panevska A, Iacovache I, Kostanjšek R, Mravinec M, Skočaj M, Zuber B, Pavšič A, Razinger J, Modic Š, Trenti F, Guella G, Sepčić K. Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm. Toxins. 2021; 13(7):455. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070455

Chicago/Turabian Style

Milijaš Jotić, Matej, Anastasija Panevska, Ioan Iacovache, Rok Kostanjšek, Martina Mravinec, Matej Skočaj, Benoît Zuber, Ana Pavšič, Jaka Razinger, Špela Modic, Francesco Trenti, Graziano Guella, and Kristina Sepčić. 2021. "Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm" Toxins 13, no. 7: 455. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070455

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop