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Dissecting Out the Molecular Mechanism of Insecticidal Activity of Ostreolysin A6/Pleurotolysin B Complexes on Western Corn Rootworm
Article

Effects of Bioinsecticidal Aegerolysin-Based Cytolytic Complexes on Non-Target Organisms

1
Department of Biology, Biotechnical Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
2
Department of Biotechnology, University of Rijeka, 51000 Rijeka, Croatia
3
Institute of Preclinical Sciences, Veterinary Faculty, University of Ljubljana, 1000 Ljubljana, Slovenia
*
Authors to whom correspondence should be addressed.
Received: 1 June 2021 / Revised: 25 June 2021 / Accepted: 28 June 2021 / Published: 30 June 2021
Aegerolysin proteins ostreolysin A6 (OlyA6), pleurotolysin A2 (PlyA2) and erylysin A (EryA) produced by the mushroom genus Pleurotus bind strongly to an invertebrate-specific membrane sphingolipid, and together with a protein partner pleurotolysin B (PlyB), form transmembrane pore complexes. This pore formation is the basis for the selective insecticidal activity of aegerolysin/PlyB complexes against two economically important coleopteran pests: the Colorado potato beetle and the western corn rootworm. In this study, we evaluated the toxicities of these aegerolysin/PlyB complexes using feeding tests with two ecologically important non-target arthropod species: the woodlouse and the honey bee. The mammalian toxicity of the EryA/PlyB complex was also evaluated after intravenous administration to mice. None of the aegerolysin/PlyB complexes were toxic against woodlice, but OlyA6/PlyB and PlyA2/PlyB were toxic to honeybees, with 48 h mean lethal concentrations (LC50) of 0.22 and 0.39 mg/mL, respectively, in their food. EryA/PlyB was also tested intravenously in mice up to 3 mg/kg body mass, without showing toxicity. With no toxicity seen for EryA/PlyB for environmentally beneficial arthropods and mammals at the tested concentrations, these EryA/PlyB complexes are of particular interest for development of new bioinsecticides for control of selected coleopteran pests. View Full-Text
Keywords: adverse effects; aegerolysins; Apis mellifera; bioinsecticides; erylysin A; MACPF protein; non-target organisms; oyster mushroom; Porcellio scaber; toxicity adverse effects; aegerolysins; Apis mellifera; bioinsecticides; erylysin A; MACPF protein; non-target organisms; oyster mushroom; Porcellio scaber; toxicity
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MDPI and ACS Style

Panevska, A.; Glavan, G.; Jemec Kokalj, A.; Kukuljan, V.; Trobec, T.; Žužek, M.C.; Vrecl, M.; Drobne, D.; Frangež, R.; Sepčić, K. Effects of Bioinsecticidal Aegerolysin-Based Cytolytic Complexes on Non-Target Organisms. Toxins 2021, 13, 457. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070457

AMA Style

Panevska A, Glavan G, Jemec Kokalj A, Kukuljan V, Trobec T, Žužek MC, Vrecl M, Drobne D, Frangež R, Sepčić K. Effects of Bioinsecticidal Aegerolysin-Based Cytolytic Complexes on Non-Target Organisms. Toxins. 2021; 13(7):457. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070457

Chicago/Turabian Style

Panevska, Anastasija, Gordana Glavan, Anita Jemec Kokalj, Veronika Kukuljan, Tomaž Trobec, Monika C. Žužek, Milka Vrecl, Damjana Drobne, Robert Frangež, and Kristina Sepčić. 2021. "Effects of Bioinsecticidal Aegerolysin-Based Cytolytic Complexes on Non-Target Organisms" Toxins 13, no. 7: 457. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13070457

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