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Article

DL-Selenomethionine Alleviates Oxidative Stress Induced by Zearalenone via Nrf2/Keap1 Signaling Pathway in IPEC-J2 Cells

Institute of Animal Nutrition, College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China
*
Authors to whom correspondence should be addressed.
Authors contributed equally to this work.
Received: 31 May 2021 / Revised: 5 August 2021 / Accepted: 6 August 2021 / Published: 10 August 2021
(This article belongs to the Special Issue Remediation Strategies for Mycotoxin in Animal Feed)
Zearalenone (ZEN) is a kind of nonsteroidal mycotoxin that is considered a risk affecting the safety of human food and livestock feed that causes oxidative damages in mammalian cells. Selenomethionine (SeMet) was indicated to have antioxidant activity and received great interest in investigating the role of SeMet as a therapeutic agent in oxidation. Therefore, the aim of this study was to investigate the hormetic role of DL-SeMet in porcine intestinal epithelial J2 (IPEC-J2) cells against ZEN-induced oxidative stress injury. As a result of this experiment, 30 μg/mL of ZEN was observed with significantly statistical effects in cell viability. Following the dose-dependent manner, 20 μg/mL was chosen for the subsequent experiments. Then, further results in the current study showed that the ZENinduced oxidative stress with subsequent suppression of the expression of antioxidant stress pathway-related genes species. Moreover, SeMet reversed the oxidative damage and cell death of ZEN toxins to some extent, by a Nrf2/Keap1-ARE pathway. The finding of this experiment provided a foundation for further research on the ZEN-caused cell oxidative damage and the cure technology. View Full-Text
Keywords: zearalenone; selenomethionine; IPEC-J2; oxidative stress; Nrf2/Keap1 signaling zearalenone; selenomethionine; IPEC-J2; oxidative stress; Nrf2/Keap1 signaling
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MDPI and ACS Style

Sun, H.; Zhang, M.; Li, J.; Shan, A. DL-Selenomethionine Alleviates Oxidative Stress Induced by Zearalenone via Nrf2/Keap1 Signaling Pathway in IPEC-J2 Cells. Toxins 2021, 13, 557. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080557

AMA Style

Sun H, Zhang M, Li J, Shan A. DL-Selenomethionine Alleviates Oxidative Stress Induced by Zearalenone via Nrf2/Keap1 Signaling Pathway in IPEC-J2 Cells. Toxins. 2021; 13(8):557. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080557

Chicago/Turabian Style

Sun, Haoyang, Meiling Zhang, Jianping Li, and Anshan Shan. 2021. "DL-Selenomethionine Alleviates Oxidative Stress Induced by Zearalenone via Nrf2/Keap1 Signaling Pathway in IPEC-J2 Cells" Toxins 13, no. 8: 557. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080557

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