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Article

Humoral Immune Response Evaluation in Horses Vaccinated with Recombinant Clostridium perfringens Toxoids Alpha and Beta for 12 Months

1
Instituto de Medicina Veterinária, Universidade Federal do Pará, Castanhal CEP 68740-970, Brazil
2
Centro de Desenvolvimento Tecnológico, Núcleo de Biotecnologia, Universidade Federal de Pelotas, Rio Grande do Sul CEP 96160-000, Brazil
3
Faculdade de Veterinária, Universidade Federal de Pelotas, Rio Grande do Sul CEP 96160-000, Brazil
*
Author to whom correspondence should be addressed.
Received: 23 June 2021 / Revised: 16 July 2021 / Accepted: 19 July 2021 / Published: 13 August 2021
In horses, Clostridium perfringens is associated with acute and fatal enterocolitis, which is caused by a beta toxin (CPB), and myonecrosis, which is caused by an alpha toxin (CPA). Although the most effective way to prevent these diseases is through vaccination, specific clostridial vaccines for horses against C. perfringens are not widely available. The aim of this study was to pioneer the immunization of horses with three different concentrations (100, 200 and 400 µg) of C. perfringens recombinant alpha (rCPA) and beta (rCPB) proteins, as well as to evaluate the humoral immune response over 360 days. Recombinant toxoids were developed and applied to 50 horses on days 0 and 30. Those vaccines attempted to stimulate the production of alpha antitoxin (anti-CPA) and beta antitoxin (anti-CPB), in addition to becoming innocuous, stable and sterile. There was a reduction in the level of neutralizing anti-CPA and anti-CPB antibodies following the 60th day; therefore, the concentrations of 200 and 400 µg capable of inducing a detectable humoral immune response were not determined until day 180. In practical terms, 200 µg is possibly the ideal concentration for use in the veterinary industry’s production of vaccines against the action of C. perfringens in equine species. View Full-Text
Keywords: recombinant alpha toxin; recombinant beta toxin; vaccine; myonecrosis; enterocolitis recombinant alpha toxin; recombinant beta toxin; vaccine; myonecrosis; enterocolitis
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MDPI and ACS Style

Freitas, N.F.Q.R.; Otaka, D.Y.; Galvão, C.C.; de Almeida, D.M.; Ferreira, M.R.A.; Moreira Júnior, C.; Hidalgo, M.M.M.H.; Conceição, F.R.; Salvarani, F.M. Humoral Immune Response Evaluation in Horses Vaccinated with Recombinant Clostridium perfringens Toxoids Alpha and Beta for 12 Months. Toxins 2021, 13, 566. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080566

AMA Style

Freitas NFQR, Otaka DY, Galvão CC, de Almeida DM, Ferreira MRA, Moreira Júnior C, Hidalgo MMMH, Conceição FR, Salvarani FM. Humoral Immune Response Evaluation in Horses Vaccinated with Recombinant Clostridium perfringens Toxoids Alpha and Beta for 12 Months. Toxins. 2021; 13(8):566. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080566

Chicago/Turabian Style

Freitas, Nayra F.Q.R., Denis Y. Otaka, Cleideanny C. Galvão, Dayane M. de Almeida, Marcos R.A. Ferreira, Clóvis Moreira Júnior, Marina M.M.H. Hidalgo, Fabricio R. Conceição, and Felipe M. Salvarani 2021. "Humoral Immune Response Evaluation in Horses Vaccinated with Recombinant Clostridium perfringens Toxoids Alpha and Beta for 12 Months" Toxins 13, no. 8: 566. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13080566

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