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Article

N-Acetylcysteine Inhibits Patulin-Induced Apoptosis by Affecting ROS-Mediated Oxidative Damage Pathway

College of Food Science and Engineering, Northwest A & F University, Yangling 712100, China
*
Author to whom correspondence should be addressed.
Jiayu Liu and Qi Liu contributed equally to this work.
Received: 17 June 2021 / Revised: 20 August 2021 / Accepted: 23 August 2021 / Published: 26 August 2021
Patulin (PAT) belongs to the family of food-borne mycotoxins. Our previous studies revealed that PAT caused cytotoxicity in human embryonic kidney cells (HEK293). In the present research, we systematically explored the detailed mechanism of ROS production and ROS clearance in PAT-induced HEK293 cell apoptosis. Results showed that PAT treatment (2.5, 5, 7.5, 10 μM) for 10 h could regulate the expression of genes and proteins involved in the mitochondrial respiratory chain complex, resulting in dysfunction of mitochondrial oxidative phosphorylation and induction of ROS overproduction. We further investigated the role of N-acetylcysteine (NAC), an ROS scavenger, in promoting the survival of PAT-treated HEK293 cells. NAC improves PAT-induced apoptosis of HEK293 cells by clearing excess ROS, modulating the expression of mitochondrial respiratory chain complex genes and proteins, and maintaining normal mitochondrial function. In addition, NAC protects the activity of antioxidant enzymes, maintains normal GSH content, and relieves oxidative damage. Additionally, 4 mM NAC alleviated 7.5 μM PAT-mediated apoptosis through the caspase pathway in HEK293 cells. In summary, our study demonstrated that ROS is significant in PAT-mediated cytotoxicity, which provides valuable insight into the management of PAT-associated health issues. View Full-Text
Keywords: PAT; apoptosis; ROS; oxidative stress; mitochondrial oxidative phosphorylation PAT; apoptosis; ROS; oxidative stress; mitochondrial oxidative phosphorylation
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MDPI and ACS Style

Liu, J.; Liu, Q.; Han, J.; Feng, J.; Guo, T.; Li, Z.; Min, F.; Jin, R.; Peng, X. N-Acetylcysteine Inhibits Patulin-Induced Apoptosis by Affecting ROS-Mediated Oxidative Damage Pathway. Toxins 2021, 13, 595. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090595

AMA Style

Liu J, Liu Q, Han J, Feng J, Guo T, Li Z, Min F, Jin R, Peng X. N-Acetylcysteine Inhibits Patulin-Induced Apoptosis by Affecting ROS-Mediated Oxidative Damage Pathway. Toxins. 2021; 13(9):595. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090595

Chicago/Turabian Style

Liu, Jiayu, Qi Liu, Jiahui Han, Jiayu Feng, Tianmin Guo, Zhiman Li, Fenyi Min, Ruyi Jin, and Xiaoli Peng. 2021. "N-Acetylcysteine Inhibits Patulin-Induced Apoptosis by Affecting ROS-Mediated Oxidative Damage Pathway" Toxins 13, no. 9: 595. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090595

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