Next Article in Journal
New Cytoplasmic Virus-Like Elements (VLEs) in the Yeast Debaryomyces hansenii
Previous Article in Journal
Evaluation of Signaling Pathways Profiling in Human Dermal Endothelial Cells Treated by Snake Venom Cysteine-Rich Secretory Proteins (svCRiSPs) from North American Snakes Using Reverse Phase Protein Array (RPPA)
Previous Article in Special Issue
Effect of Different Species of Prorocentrum Genus on the Japanese Oyster Crassostrea gigas Proteomic Profile
Article

Expression Analyses of Genes Related to Multixenobiotic Resistance in Mytilus galloprovincialis after Exposure to Okadaic Acid-Producing Dinophysis acuminata

1
Departamento de Bioquímica y Biología Molecular, Instituto de Acuicultura, Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain
2
Centro de Investigacións Mariñas, Xunta de Galicia, Pedras de Corón s/n Apdo. 13, 36620 Vilanova de Arousa, Spain
*
Author to whom correspondence should be addressed.
Received: 9 June 2021 / Revised: 26 August 2021 / Accepted: 27 August 2021 / Published: 1 September 2021
(This article belongs to the Special Issue Omic Technologies Applied to the Study of Marine Shellfish Toxins)
The mussel Mytilus galloprovincialis is one of the most important aquaculture species in Europe. Its main production problem is the accumulation of toxins during coastal blooms, which prevents mussel commercialization. P-glycoprotein (ABCB1/MDR1/P-gp) is part of the multixenobiotic resistance system in aquatic organisms, and okadaic acid, the main DSP toxin, is probably a substrate of the P-gp-mediated efflux. In this study, the presence and possible role of P-gp in the okadaic acid detoxification process was studied in M. galloprovincialis. We identified, cloned, and characterized two complete cDNAs of mdr1 and mdr2 genes. MgMDR1 and MgMDR2 predicted proteins had the structure organization of ABCB full transporters, and were identified as P-gp/MDR/ABCB proteins. Furthermore, the expression of mdr genes was monitored in gills, digestive gland, and mantle during a cycle of accumulation-elimination of okadaic acid. Mdr1 significantly increased its expression in the digestive gland and gills, supporting the idea of an important role of the MDR1 protein in okadaic acid efflux out of cells in these tissues. The expression of M. galloprovincialismrp2, a multidrug associated protein (MRP/ABCC), was also monitored. As in the case of mdr1, there was a significant induction in the expression of mrp2 in the digestive gland, as the content of okadaic acid increased. Thus, P-gp and MRP might constitute a functional defense network against xenobiotics, and might be involved in the resistance mechanisms to DSP toxins. View Full-Text
Keywords: P-glycoprotein; mdr; mrp; ABC transporters; DSP toxins; MXR P-glycoprotein; mdr; mrp; ABC transporters; DSP toxins; MXR
Show Figures

Graphical abstract

MDPI and ACS Style

Martínez-Escauriaza, R.; Lozano, V.; Pérez-Parallé, M.L.; Blanco, J.; Sánchez, J.L.; Pazos, A.J. Expression Analyses of Genes Related to Multixenobiotic Resistance in Mytilus galloprovincialis after Exposure to Okadaic Acid-Producing Dinophysis acuminata. Toxins 2021, 13, 614. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090614

AMA Style

Martínez-Escauriaza R, Lozano V, Pérez-Parallé ML, Blanco J, Sánchez JL, Pazos AJ. Expression Analyses of Genes Related to Multixenobiotic Resistance in Mytilus galloprovincialis after Exposure to Okadaic Acid-Producing Dinophysis acuminata. Toxins. 2021; 13(9):614. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090614

Chicago/Turabian Style

Martínez-Escauriaza, Roi, Vanessa Lozano, M. L. Pérez-Parallé, Juan Blanco, José L. Sánchez, and Antonio J. Pazos 2021. "Expression Analyses of Genes Related to Multixenobiotic Resistance in Mytilus galloprovincialis after Exposure to Okadaic Acid-Producing Dinophysis acuminata" Toxins 13, no. 9: 614. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins13090614

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop