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Article

A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS)

1
Department of Community Health Promotion, Tohoku University Graduate School of Medicine, Sendai 980-8574, Japan
2
Division of Nephrology, Endocrinology and Vascular Medicine, Tohoku University Graduate School of Medicine, Sendai 980-8574, Japan
3
Department of Clinical Biology and Hormonal Regulation, Tohoku University Graduate School of Medicine, Sendai 980-8574, Japan
4
Department of Preventive Medicine and Epidemiology, Tohoku Medical Megabank Organization, Tohoku University, Sendai 980-8573, Japan
5
Institute for Advanced Biosciences, Keio University, Tsuruoka, Yamagata 997-0052, Japan
6
Division of Medical Science, Tohoku University Graduate School of Biomedical Engineering, Sendai 980-8574, Japan
*
Author to whom correspondence should be addressed.
Received: 18 July 2012 / Revised: 19 September 2012 / Accepted: 26 October 2012 / Published: 14 November 2012
(This article belongs to the Special Issue Uremic Toxins)
The oral adsorbent AST-120 is composed of spherical carbon particles and has an adsorption ability for certain small-molecular-weight compounds that accumulate in patients with chronic kidney disease (CKD). So far, very few compounds are known to be adsorbed by AST-120 in vivo. To examine the effect of AST-120 in vivo, we comprehensively evaluated the plasma concentrations of 146 compounds (61 anions and 85 cations) in CKD model rats, with or without four weeks of treatment with AST-120. By capillary electrophoresis with mass spectrometry, we identified 6 anions and 17 cations that were significantly decreased by AST-120 treatment. In contrast, we also identified 2 cations that were significantly increased by AST-120. Among them, 4 anions, apart from indoxyl sulfate and hippurate, and 19 cations were newly identified in this study. The plasma levels of N-acetyl-neuraminate, 4-pyridoxate, 4-oxopentanoate, glycine, γ-guanidinobutyrate, N-γ-ethylglutamine, allantoin, cytosine, 5-methylcytosine and imidazole-4-acetate were significantly increased in the CKD model compared with the sham-operated group, and were significantly decreased by AST-120 treatment. Therefore, these 10 compounds could be added as uremic compounds that indicate the effect of AST-120 treatment. This study provides useful information not only for identifying the indicators of AST-120, but also for clarifying changes in the metabolic profile by AST-120 treatment in the clinical setting. View Full-Text
Keywords: AST-120; uremic toxin; CE-MS AST-120; uremic toxin; CE-MS
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MDPI and ACS Style

Akiyama, Y.; Takeuchi, Y.; Kikuchi, K.; Mishima, E.; Yamamoto, Y.; Suzuki, C.; Toyohara, T.; Suzuki, T.; Hozawa, A.; Ito, S.; Soga, T.; Abe, T. A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS). Toxins 2012, 4, 1309-1322. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins4111309

AMA Style

Akiyama Y, Takeuchi Y, Kikuchi K, Mishima E, Yamamoto Y, Suzuki C, Toyohara T, Suzuki T, Hozawa A, Ito S, Soga T, Abe T. A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS). Toxins. 2012; 4(11):1309-1322. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins4111309

Chicago/Turabian Style

Akiyama, Yasutoshi, Yoichi Takeuchi, Koichi Kikuchi, Eikan Mishima, Yasuaki Yamamoto, Chitose Suzuki, Takafumi Toyohara, Takehiro Suzuki, Atsushi Hozawa, Sadayoshi Ito, Tomoyoshi Soga, and Takaaki Abe. 2012. "A Metabolomic Approach to Clarifying the Effect of AST-120 on 5/6 Nephrectomized Rats by Capillary Electrophoresis with Mass Spectrometry (CE-MS)" Toxins 4, no. 11: 1309-1322. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins4111309

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