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Multimodal Protein Constructs for Herbivore Insect Control

Département de phytologie, Centre de recherche en horticulture, Pavillon des Services, Université Laval, Québec QC, G1V 0A6, Canada
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Received: 8 March 2012 / Revised: 1 June 2012 / Accepted: 5 June 2012 / Published: 12 June 2012
(This article belongs to the Special Issue Insecticidal Toxins)
Transgenic plants expressing combinations of microbial or plant pesticidal proteins represent a promising tool for the efficient, durable control of herbivorous insects. In this review we describe current strategies devised for the heterologous co-expression of pesticidal proteins in planta, some of which have already shown usefulness in plant protection. Emphasis is placed on protein engineering strategies involving the insertion of single DNA constructs within the host plant genome. Multimodal fusion proteins integrating complementary pesticidal functions along a unique polypeptide are first considered, taking into account the structural constraints associated with protein or protein domain grafting to biologically active proteins. Strategies that allow for the co- or post-translational release of two or more pesticidal proteins are then considered, including polyprotein precursors releasing free proteins upon proteolytic cleavage, and multicistronic transcripts for the parallel translation of single protein-encoding mRNA sequences. View Full-Text
Keywords: insect-resistant transgenic plants; Bt plants; Cry toxins; defense proteins; gene stacking; polyproteins; protein pyramiding; fusion proteins insect-resistant transgenic plants; Bt plants; Cry toxins; defense proteins; gene stacking; polyproteins; protein pyramiding; fusion proteins
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MDPI and ACS Style

Sainsbury, F.; Benchabane, M.; Goulet, M.-C.; Michaud, D. Multimodal Protein Constructs for Herbivore Insect Control. Toxins 2012, 4, 455-475. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins4060455

AMA Style

Sainsbury F, Benchabane M, Goulet M-C, Michaud D. Multimodal Protein Constructs for Herbivore Insect Control. Toxins. 2012; 4(6):455-475. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins4060455

Chicago/Turabian Style

Sainsbury, Frank, Meriem Benchabane, Marie-Claire Goulet, and Dominique Michaud. 2012. "Multimodal Protein Constructs for Herbivore Insect Control" Toxins 4, no. 6: 455-475. https://0-doi-org.brum.beds.ac.uk/10.3390/toxins4060455

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