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Article

A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue

by 1,†, 1,†, 2,†, 1 and 1,3,4,*
1
Department of Mechano-Informatics, Graduate School of Information Science and Technology, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
2
Department of Mechano-Informatics, Faculty of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
3
Institute of Industrial Science (IIS), The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505, Japan
4
International Research Center for Neurointelligence (WPI-IRCN), The University of Tokyo Institutes for Advanced Study, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
*
Author to whom correspondence should be addressed.
Contributed equally.
Academic Editor: Nam-Trung Nguyen
Micromachines 2021, 12(11), 1411; https://0-doi-org.brum.beds.ac.uk/10.3390/mi12111411
Received: 14 October 2021 / Revised: 13 November 2021 / Accepted: 14 November 2021 / Published: 17 November 2021
(This article belongs to the Special Issue Feature Papers of Micromachines in Biology and Biomedicine 2021)
Muscle tissues can be fabricated in vitro by culturing myoblast-populated hydrogels. To counter the shrinkage of the myoblast-populated hydrogels during culture, a pair of anchors are generally utilized to fix the two ends of the hydrogel. Here, we propose an alternative method to counter the shrinkage of the hydrogel and fabricate plane-shaped skeletal muscle tissues. The method forms myoblast-populated hydrogel in a cylindrical cavity with a central pillar, which can prevent tissue shrinkage along the circumferential direction. By eliminating the usages of the anchor pairs, our proposed method can produce plane-shaped skeletal muscle tissues with uniform width and thickness. In experiments, we demonstrate the fabrication of plane-shaped (length: ca. 10 mm, width: 5~15 mm) skeletal muscle tissue with submillimeter thickness. The tissues have uniform shapes and are populated with differentiated muscle cells stained positive for myogenic differentiation markers (i.e., myosin heavy chains). In addition, we show the assembly of subcentimeter-order tissue blocks by stacking the plane-shaped skeletal muscle tissues. The proposed method can be further optimized and scaled up to produce cultured animal products such as cultured meat. View Full-Text
Keywords: biofabrication; tissue engineering; skeletal muscle; microtissue assembly biofabrication; tissue engineering; skeletal muscle; microtissue assembly
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MDPI and ACS Style

Nie, M.; Shima, A.; Fukushima, K.; Morimoto, Y.; Takeuchi, S. A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue. Micromachines 2021, 12, 1411. https://0-doi-org.brum.beds.ac.uk/10.3390/mi12111411

AMA Style

Nie M, Shima A, Fukushima K, Morimoto Y, Takeuchi S. A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue. Micromachines. 2021; 12(11):1411. https://0-doi-org.brum.beds.ac.uk/10.3390/mi12111411

Chicago/Turabian Style

Nie, Minghao, Ai Shima, Kenta Fukushima, Yuya Morimoto, and Shoji Takeuchi. 2021. "A Cylindrical Molding Method for the Biofabrication of Plane-Shaped Skeletal Muscle Tissue" Micromachines 12, no. 11: 1411. https://0-doi-org.brum.beds.ac.uk/10.3390/mi12111411

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