Biosynthesis of Pyrrole-2-carbaldehyde via Enzymatic CO2 Fixation
Manchester Institute of Biotechnology, University of Manchester, 131 Princess Street, Manchester M1 7DN, UK
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Author to whom correspondence should be addressed.
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Current address: Chemistry Research Laboratory, University of Oxford, Oxford OX1 3TA, UK.
Academic Editors: Kuan Chang and Consuelo Alvarez-Galvan
Catalysts 2022, 12(5), 538; https://0-doi-org.brum.beds.ac.uk/10.3390/catal12050538
Received: 15 March 2022
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Revised: 29 April 2022
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Accepted: 6 May 2022
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Published: 14 May 2022
(This article belongs to the Special Issue CO2 Catalytic Conversion and Utilization)
The use of CO2 as a chemical building block is of considerable interest. To achieve carbon fixation at ambient conditions, (de)carboxylase enzymes offer an attractive route but frequently require elevated [CO2] levels to yield the acid product. However, it has recently been shown that the coupling of a UbiD-type decarboxylase with carboxylic acid reductase yields the corresponding aldehyde product at near ambient [CO2]. Here, we show this approach can be expanded to different UbiD and CAR enzymes to yield alternative products, in this case, the production of pyrrole-2-carbaldehyde from pyrrole, using Pseudomonas aeruginosa HudA/PA0254 in combination with Segniliparus rotundus CAR. This confirms the varied substrate range of the respective UbiD and CAR enzymes can be harnessed in distinct combinations to support production of a wide range of aldehydes via enzymatic CO2 fixation.
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Keywords:
CO2 fixation; biocatalysts; UbiD family (de)carboxylases; carboxylic acid reductases; biocatalytic cascades
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MDPI and ACS Style
Titchiner, G.R.; Marshall, S.A.; Miscikas, H.; Leys, D. Biosynthesis of Pyrrole-2-carbaldehyde via Enzymatic CO2 Fixation. Catalysts 2022, 12, 538. https://0-doi-org.brum.beds.ac.uk/10.3390/catal12050538
AMA Style
Titchiner GR, Marshall SA, Miscikas H, Leys D. Biosynthesis of Pyrrole-2-carbaldehyde via Enzymatic CO2 Fixation. Catalysts. 2022; 12(5):538. https://0-doi-org.brum.beds.ac.uk/10.3390/catal12050538
Chicago/Turabian StyleTitchiner, Gabriel R., Stephen A. Marshall, Herkus Miscikas, and David Leys. 2022. "Biosynthesis of Pyrrole-2-carbaldehyde via Enzymatic CO2 Fixation" Catalysts 12, no. 5: 538. https://0-doi-org.brum.beds.ac.uk/10.3390/catal12050538
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