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Review

Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside

1
NanoBioCel Group, University of the Basque Country, Vitoria-Gasteiz, 01006, Spain
2
Networking Research Center of Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Zaragoza, 50018, Spain
3
Institute of Analytical Sciences and Physico-Chemistry for Environment and Materials (IPREM) (UMR CNRS 5254), University of Pau and Adour Countries, Hélioparc Pau Pyrénées, Pau, 64000, France
*
Author to whom correspondence should be addressed.
Received: 14 February 2014 / Revised: 21 May 2014 / Accepted: 26 May 2014 / Published: 16 June 2014
(This article belongs to the Collection Polysaccharides)
Non-viral gene delivery vectors are emerging as a safer alternative to viral vectors. Among natural polymers, chitosan (Ch) is the most studied one, and low molecular weight Ch, specifically, presents a wide range of advantages for non-viral pDNA delivery. It is crucial to determine the best process for the formation of Low Molecular Weight Chitosan (LMWC)-pDNA complexes and to characterize their physicochemical properties to better understand their behavior once the polyplexes are administered. The transfection efficiency of Ch based polyplexes is relatively low. Therefore, it is essential to understand all the transfection process, including the cellular uptake, endosomal escape and nuclear import, together with the parameters involved in the process to improve the design and development of the non-viral vectors. The aim of this review is to describe the formation and characterization of LMWC based polyplexes, the in vitro transfection process and finally, the in vivo applications of LMWC based polyplexes for gene therapy purposes. View Full-Text
Keywords: low molecular weight chitosan (LMWC); pDNA; polyplex; non-viral vector; gene delivery; transfection efficiency low molecular weight chitosan (LMWC); pDNA; polyplex; non-viral vector; gene delivery; transfection efficiency
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MDPI and ACS Style

Agirre, M.; Zarate, J.; Ojeda, E.; Puras, G.; Desbrieres, J.; Pedraz, J.L. Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside. Polymers 2014, 6, 1727-1755. https://0-doi-org.brum.beds.ac.uk/10.3390/polym6061727

AMA Style

Agirre M, Zarate J, Ojeda E, Puras G, Desbrieres J, Pedraz JL. Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside. Polymers. 2014; 6(6):1727-1755. https://0-doi-org.brum.beds.ac.uk/10.3390/polym6061727

Chicago/Turabian Style

Agirre, Mireia, Jon Zarate, Edilberto Ojeda, Gustavo Puras, Jacques Desbrieres, and Jose L. Pedraz. 2014. "Low Molecular Weight Chitosan (LMWC)-based Polyplexes for pDNA Delivery: From Bench to Bedside" Polymers 6, no. 6: 1727-1755. https://0-doi-org.brum.beds.ac.uk/10.3390/polym6061727

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