Hes5.9 Coordinate FGF and Notch Signaling to Modulate Gastrulation via Regulating Cell Fate Specification and Cell Migration in Xenopus tropicalis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Ethics and Embryo Manipulation
2.2. Multiple Sequence Alignment and Phylogenetic Tree Construction
2.3. RNA Extraction, Reverse Transcription-PCR, and cDNA Cloning
2.4. mRNA Synthesis and Microinjection
2.5. Quantitative Reverse Transcription PCR (RT-qPCR)
2.6. Whole-Mount in situ Hybridization
2.7. Animal Cap and Dorsal Marginal Zone (DMZ) Elongation Assays
2.8. RNA-Sequencing and Data Analyses
3. Results
3.1. Hes5.9 is Regulated by the Notch Signaling
3.2. Spatiotemporal Expression Pattern of Hes5.9 in Embryonic Development
3.3. The FGF Signaling is Required for the Spatiotemporal Expression Pattern of Hes5.9 during Gastrulation
3.4. Knockdown of Hes5.9 Results in Defects on Gastrulation
3.5. Overexpression of Hes5.9 Causes Defects on Gastrulation
3.6. Hes5.9 Modulates the Elongation of Animal Cap and DMZ Explants
3.7. Transcriptomics Analysis of Hes5.9 Ectopic Expression in Late Gastrula Stage
4. Discussion and Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Huang, X.; Zhang, L.; Yang, S.; Zhang, Y.; Wu, M.; Chen, P. Hes5.9 Coordinate FGF and Notch Signaling to Modulate Gastrulation via Regulating Cell Fate Specification and Cell Migration in Xenopus tropicalis. Genes 2020, 11, 1363. https://0-doi-org.brum.beds.ac.uk/10.3390/genes11111363
Huang X, Zhang L, Yang S, Zhang Y, Wu M, Chen P. Hes5.9 Coordinate FGF and Notch Signaling to Modulate Gastrulation via Regulating Cell Fate Specification and Cell Migration in Xenopus tropicalis. Genes. 2020; 11(11):1363. https://0-doi-org.brum.beds.ac.uk/10.3390/genes11111363
Chicago/Turabian StyleHuang, Xiao, Liyue Zhang, Shanshan Yang, Yongpu Zhang, Mingjiang Wu, and Peichao Chen. 2020. "Hes5.9 Coordinate FGF and Notch Signaling to Modulate Gastrulation via Regulating Cell Fate Specification and Cell Migration in Xenopus tropicalis" Genes 11, no. 11: 1363. https://0-doi-org.brum.beds.ac.uk/10.3390/genes11111363