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Article

Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport

1
RIKEN Center for Sustainable Resource Science, Kanagawa 230-0045, Japan
2
Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara 630-0192, Japan
*
Author to whom correspondence should be addressed.
Academic Editors: Sonia Gazzarrini and Eiji Nambara
Received: 7 May 2021 / Revised: 31 May 2021 / Accepted: 4 June 2021 / Published: 8 June 2021
The plant hormone abscisic acid (ABA) is actively synthesized in vascular tissues and transported to guard cells to promote stomatal closure. Although several transmembrane ABA transporters have been identified, how the movement of ABA within plants is regulated is not fully understood. In this study, we determined that Arabidopsis NPF4.6, previously identified as an ABA transporter expressed in vascular tissues, is also present in guard cells and positively regulates stomatal closure in leaves. We also found that mutants defective in NPF5.1 had a higher leaf surface temperature compared to the wild type. Additionally, NPF5.1 mediated cellular ABA uptake when expressed in a heterologous yeast system. Promoter activities of NPF5.1 were detected in several leaf cell types. Taken together, these observations indicate that NPF5.1 negatively regulates stomatal closure by regulating the amount of ABA that can be transported from vascular tissues to guard cells. View Full-Text
Keywords: abscisic acid (ABA); Arabidopsis thaliana L.; guard cells; NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF); transporter abscisic acid (ABA); Arabidopsis thaliana L.; guard cells; NITRATE TRANSPORTER 1/PEPTIDE TRANSPORTER FAMILY (NPF); transporter
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MDPI and ACS Style

Shimizu, T.; Kanno, Y.; Suzuki, H.; Watanabe, S.; Seo, M. Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport. Genes 2021, 12, 885. https://0-doi-org.brum.beds.ac.uk/10.3390/genes12060885

AMA Style

Shimizu T, Kanno Y, Suzuki H, Watanabe S, Seo M. Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport. Genes. 2021; 12(6):885. https://0-doi-org.brum.beds.ac.uk/10.3390/genes12060885

Chicago/Turabian Style

Shimizu, Takafumi, Yuri Kanno, Hiromi Suzuki, Shunsuke Watanabe, and Mitsunori Seo. 2021. "Arabidopsis NPF4.6 and NPF5.1 Control Leaf Stomatal Aperture by Regulating Abscisic Acid Transport" Genes 12, no. 6: 885. https://0-doi-org.brum.beds.ac.uk/10.3390/genes12060885

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