Next Article in Journal
Genome-Wide Identification and Characterization of Long Non-Coding RNAs from Mulberry (Morus notabilis) RNA-seq Data
Previous Article in Journal
Impact of DCC (rs714) and PSCA (rs2294008 and rs2976392) Gene Polymorphism in Modulating Cancer Risk in Asian Population
Article

Pseudo-Reference-Based Assembly of Vertebrate Transcriptomes

by 1,†, 2,† and 1,3,*
1
Department of Life Science, College of Natural Sciences, Hanyang University, Seoul 04763, Korea
2
College of Liberal Studies, Seoul National University, Seoul 08826, Korea
3
Research Institute for Natural Sciences, Hanyang University, Seoul 04763, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Montserrat Corominas
Received: 24 December 2015 / Revised: 5 February 2016 / Accepted: 17 February 2016 / Published: 24 February 2016
(This article belongs to the Section Technologies and Resources for Genetics)
High-throughput RNA sequencing (RNA-seq) provides a comprehensive picture of the transcriptome, including the identity, structure, quantity, and variability of expressed transcripts in cells, through the assembly of sequenced short RNA-seq reads. Although the reference-based approach guarantees the high quality of the resulting transcriptome, this approach is only applicable when the relevant reference genome is present. Here, we developed a pseudo-reference-based assembly (PRA) that reconstructs a transcriptome based on a linear regression function of the optimized mapping parameters and genetic distances of the closest species. Using the linear model, we reconstructed transcriptomes of four different aves, the white leg horn, turkey, duck, and zebra finch, with the Gallus gallus genome as a pseudo-reference, and of three primates, the chimpanzee, gorilla, and macaque, with the human genome as a pseudo-reference. The resulting transcriptomes show that the PRAs outperformed the de novo approach for species with within about 10% mutation rate among orthologous transcriptomes, enough to cover distantly related species as far as chicken and duck. Taken together, we suggest that the PRA method can be used as a tool for reconstructing transcriptome maps of vertebrates whose genomes have not yet been sequenced. View Full-Text
Keywords: transcriptome assembly; RNA-seq; pseudo-reference transcriptome assembly; RNA-seq; pseudo-reference
Show Figures

Figure 1

MDPI and ACS Style

Nam, K.; Jeong, H.; Nam, J.-W. Pseudo-Reference-Based Assembly of Vertebrate Transcriptomes. Genes 2016, 7, 10. https://0-doi-org.brum.beds.ac.uk/10.3390/genes7030010

AMA Style

Nam K, Jeong H, Nam J-W. Pseudo-Reference-Based Assembly of Vertebrate Transcriptomes. Genes. 2016; 7(3):10. https://0-doi-org.brum.beds.ac.uk/10.3390/genes7030010

Chicago/Turabian Style

Nam, Kyoungwoo, Heesu Jeong, and Jin-Wu Nam. 2016. "Pseudo-Reference-Based Assembly of Vertebrate Transcriptomes" Genes 7, no. 3: 10. https://0-doi-org.brum.beds.ac.uk/10.3390/genes7030010

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop