Next Article in Journal
A Multi-Step miRNA-mRNA Regulatory Network Construction Approach Identifies Gene Signatures Associated with Endometrioid Endometrial Carcinoma
Previous Article in Journal
Wool Keratin-Associated Protein Genes in Sheep—A Review
Technical Note

Selection and Verification of Candidate Reference Genes for Mature MicroRNA Expression by Quantitative RT-PCR in the Tea Plant (Camellia sinensis)

State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, 130 Changjiang West Road, Hefei 230036, China
*
Author to whom correspondence should be addressed.
Academic Editors: Lukas Mueller and Peter W. Young
Received: 27 February 2016 / Revised: 11 April 2016 / Accepted: 24 May 2016 / Published: 28 May 2016
(This article belongs to the Section Population and Evolutionary Genetics and Genomics)
Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) is a rapid and sensitive method for analyzing microRNA (miRNA) expression. However, accurate qRT-PCR results depend on the selection of reliable reference genes as internal positive controls. To date, few studies have identified reliable reference genes for differential expression analysis of miRNAs among tissues, and among experimental conditions in plants. In this study, three miRNAs and four non-coding small RNAs (ncRNA) were selected as reference candidates, and the stability of their expression was evaluated among different tissues and under different experimental conditions in the tea plant (Camellia sinensis) using the geNorm and NormFinder programs. It was shown that miR159a was the best single reference gene in the bud to the fifth leaf, 5S rRNA was the most suitable gene in different organs, miR6149 was the most stable gene when the leaves were attacked by Ectropis oblique and U4, miR5368n and miR159a were the best genes when the leaves were treated by methyl jasmonate (MeJA), salicylic acid (SA) and abscisic acid (ABA), respectively. Our results provide suitable reference genes for future investigations on miRNA functions in tea plants. View Full-Text
Keywords: Camellia sinensis; microRNA; candidate reference gene; qRT-PCR; tea plant; expression Camellia sinensis; microRNA; candidate reference gene; qRT-PCR; tea plant; expression
Show Figures

Figure 1

MDPI and ACS Style

Song, H.; Zhang, X.; Shi, C.; Wang, S.; Wu, A.; Wei, C. Selection and Verification of Candidate Reference Genes for Mature MicroRNA Expression by Quantitative RT-PCR in the Tea Plant (Camellia sinensis). Genes 2016, 7, 25. https://0-doi-org.brum.beds.ac.uk/10.3390/genes7060025

AMA Style

Song H, Zhang X, Shi C, Wang S, Wu A, Wei C. Selection and Verification of Candidate Reference Genes for Mature MicroRNA Expression by Quantitative RT-PCR in the Tea Plant (Camellia sinensis). Genes. 2016; 7(6):25. https://0-doi-org.brum.beds.ac.uk/10.3390/genes7060025

Chicago/Turabian Style

Song, Hui, Xiao Zhang, Cong Shi, Shuangshuang Wang, Ailin Wu, and Chaoling Wei. 2016. "Selection and Verification of Candidate Reference Genes for Mature MicroRNA Expression by Quantitative RT-PCR in the Tea Plant (Camellia sinensis)" Genes 7, no. 6: 25. https://0-doi-org.brum.beds.ac.uk/10.3390/genes7060025

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop