DMRTC2, PAX7, BRACHYURY/T and TERT Are Implicated in Male Germ Cell Development Following Curative Hormone Treatment for Cryptorchidism-Induced Infertility
Cryptorchidism Research Institute, Kindermedizinisches Zentrum Liestal, 4410 Liestal, Switzerland
Children’s Surgery Centre, Faculty of Medicine, Vilnius of University, Vilnius 01513, Lithuania
Biozentrum, Life Sciences Training Facility, University of Basel, 4001 Basel, Switzerland
Institute for Pathology, National Centre of Pathology, Affiliate of Vilnius University Hospital Santariskiu Klinikos, 08406 Vilnius, Lithuania
Friedrich Miescher Institute for Biomedical Research, 4058 Basel, Switzerland
Swiss Institute of Bioinformatics, Basel, Switzerland
Author to whom correspondence should be addressed.
Academic Editor: Paolo Cinelli
Genes 2017, 8(10), 267; https://0-doi-org.brum.beds.ac.uk/10.3390/genes8100267
Received: 18 August 2017 / Revised: 25 September 2017 / Accepted: 5 October 2017 / Published: 11 October 2017
(This article belongs to the Section Human Genomics and Genetic Diseases)
Defective mini-puberty results in insufficient testosterone secretion that impairs the differentiation of gonocytes into dark-type (Ad) spermatogonia. The differentiation of gonocytes into Ad spermatogonia can be induced by administration of the gonadotropin-releasing hormone agonist, GnRHa (Buserelin, INN)). Nothing is known about the mechanism that underlies successful GnRHa treatment in the germ cells. Using RNA-sequencing of testicular biopsies, we recently examined RNA profiles of testes with and without GnRHa treatment. Here, we focused on the expression patterns of known gene markers for gonocytes and spermatogonia, and found that DMRTC2, PAX7, BRACHYURY/T, and TERT were associated with defective mini-puberty and were responsive to GnRHa. These results indicate novel testosterone-dependent genes and provide valuable insight into the transcriptional response to both defective mini-puberty and curative GnRHa treatment, which prevents infertility in man with one or both undescended (cryptorchid) testes.