Next Article in Journal
Novel Insights into Antiviral Gene Regulation of Red Swamp Crayfish, Procambarus clarkii, Infected with White Spot Syndrome Virus
Next Article in Special Issue
Flanking Variation Influences Rates of Stutter in Simple Repeats
Previous Article in Journal
Next Generation Sequencing of Chromosome-Specific Libraries Sheds Light on Genome Evolution in Paleotetraploid Sterlet (Acipenser ruthenus)
Article

Human Organ Tissue Identification by Targeted RNA Deep Sequencing to Aid the Investigation of Traumatic Injury

by 1 and 1,2,*
1
National Center for Forensic Science, University of Central Florida, Orlando, FL 32816, USA
2
Department of Chemistry, University of Central Florida, Orlando, FL 32816, USA
*
Author to whom correspondence should be addressed.
Received: 17 October 2017 / Revised: 3 November 2017 / Accepted: 6 November 2017 / Published: 10 November 2017
(This article belongs to the Special Issue Forensic Genomics)
Molecular analysis of the RNA transcriptome from a putative tissue fragment should permit the assignment of its source to a specific organ, since each will exhibit a unique pattern of gene expression. Determination of the organ source of tissues from crime scenes may aid in shootings and other investigations. We have developed a prototype massively parallel sequencing (MPS) mRNA profiling assay for organ tissue identification that is designed to definitively identify 10 organ/tissue types using a targeted panel of 46 mRNA biomarkers. The identifiable organs and tissues include brain, lung, liver, heart, kidney, intestine, stomach, skeletal muscle, adipose, and trachea. The biomarkers were chosen after iterative specificity testing of numerous candidate genes in various tissue types. The assay is very specific, with little cross-reactivity with non-targeted tissue, and can detect RNA mixtures from different tissues. We also demonstrate the ability of the assay to successful identify the tissue source of origin using a single blind study. View Full-Text
Keywords: massively parallel sequencing; human organ tissue; mRNA; tissue identification; forensic science massively parallel sequencing; human organ tissue; mRNA; tissue identification; forensic science
Show Figures

Figure 1

MDPI and ACS Style

Hanson, E.; Ballantyne, J. Human Organ Tissue Identification by Targeted RNA Deep Sequencing to Aid the Investigation of Traumatic Injury. Genes 2017, 8, 319. https://0-doi-org.brum.beds.ac.uk/10.3390/genes8110319

AMA Style

Hanson E, Ballantyne J. Human Organ Tissue Identification by Targeted RNA Deep Sequencing to Aid the Investigation of Traumatic Injury. Genes. 2017; 8(11):319. https://0-doi-org.brum.beds.ac.uk/10.3390/genes8110319

Chicago/Turabian Style

Hanson, Erin, and Jack Ballantyne. 2017. "Human Organ Tissue Identification by Targeted RNA Deep Sequencing to Aid the Investigation of Traumatic Injury" Genes 8, no. 11: 319. https://0-doi-org.brum.beds.ac.uk/10.3390/genes8110319

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop