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Review

Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries

IRCM, Institut de Recherche en Cancérologie de Montpellier, INSERM U1194, Université de Montpellier, F-34298 Montpellier, France
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Received: 28 October 2020 / Revised: 15 December 2020 / Accepted: 7 January 2021 / Published: 14 January 2021
Immunohistochemistry is a widely used technique for research and diagnostic purposes that relies on the recognition by antibodies of antigens expressed in tissues. However, tissue processing and particularly formalin fixation affect the conformation of these antigens through the formation of methylene bridges. Although antigen retrieval techniques can partially restore antigen immunoreactivity, it is difficult to identify antibodies that can recognize their target especially in formalin-fixed paraffin-embedded tissues. Most of the antibodies currently used in immunohistochemistry have been obtained by animal immunization; however, in vitro display techniques represent alternative strategies that have not been fully explored yet. This review provides an overview of phage display-based antibody selections using naïve antibody libraries on various supports (fixed cells, dissociated tissues, tissue fragments, and tissue sections) that have led to the identification of antibodies suitable for immunohistochemistry. View Full-Text
Keywords: immunohistochemistry; phage display; monoclonal antibody immunohistochemistry; phage display; monoclonal antibody
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MDPI and ACS Style

Mairaville, C.; Martineau, P. Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries. Antibodies 2021, 10, 4. https://0-doi-org.brum.beds.ac.uk/10.3390/antib10010004

AMA Style

Mairaville C, Martineau P. Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries. Antibodies. 2021; 10(1):4. https://0-doi-org.brum.beds.ac.uk/10.3390/antib10010004

Chicago/Turabian Style

Mairaville, Célestine, and Pierre Martineau. 2021. "Antibody Identification for Antigen Detection in Formalin-Fixed Paraffin-Embedded Tissue Using Phage Display and Naïve Libraries" Antibodies 10, no. 1: 4. https://0-doi-org.brum.beds.ac.uk/10.3390/antib10010004

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